Spectroscopic Evidence for Polymorphic Aggregates Formed by Amyloid-beta Fragments
文献类型：期刊论文
| 作者 | Guo, Yangmei; Wang, Jianping |
| 刊名 | CHEMPHYSCHEM
 |
| 出版日期 | 2012-12-07 |
| 卷号 | 13期号:17页码:3901-3908 |
| 关键词 | Amyloid Beta-peptides Ir Spectroscopy Aggregation Electron Microscopy Protein Structures |
| ISSN号 | 1439-4235 |
| DOI | 10.1002/cphc.201200611 |
| 英文摘要 | Understanding the structure of amyloid-beta (A beta) aggregates is a key step towards elucidating the pathology of Alzheimers disease. In this work, three fragments of the A beta 142 protein, A beta 125 (DAEFRHDSGYEVHHQKLVFFAEDVG), A beta 2535 (GSNKGAIIGLM), and A beta 3342 (GLMVGGVVIA), were synthesized, and their aggregated structures were examined by linear infrared spectroscopy in the amide-I (mainly the C?O stretching) region. The structures of the formed aggregates were found to be both sequence and pH dependent. The results suggest that instead of forming matured fibrils, as in the case of full-length A beta 142, both A beta 125 and A beta 3342 form a mixture of threadlike beta-sheet fibril, soluble beta-sheet oligomer, and random coil structures. The beta-sheet conformations were found to be mainly antiparallel for the former and both parallel and antiparallel for the latter. However, the A beta 2535 fragment was found to form assembled fibrils containing predominantly parallel beta-sheets. The conformation and morphology of the aggregates were also confirmed by circular dichroism measurements and transmission electron microscopy. Factors influencing the structures of the aggregates formed by the A beta fragments were discussed. |
| 语种 | 英语 |
| WOS记录号 | WOS:000312038200022 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49757]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Jianping |
| 作者单位 | Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Yangmei,Wang, Jianping. Spectroscopic Evidence for Polymorphic Aggregates Formed by Amyloid-beta Fragments[J]. CHEMPHYSCHEM,2012,13(17):3901-3908. |
| APA | Guo, Yangmei,&Wang, Jianping.(2012).Spectroscopic Evidence for Polymorphic Aggregates Formed by Amyloid-beta Fragments.CHEMPHYSCHEM,13(17),3901-3908. |
| MLA | Guo, Yangmei,et al."Spectroscopic Evidence for Polymorphic Aggregates Formed by Amyloid-beta Fragments".CHEMPHYSCHEM 13.17(2012):3901-3908. |
入库方式： OAI收割
来源：化学研究所
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