Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays
文献类型：期刊论文
| 作者 | Ha, Qing1,2,3; Yang, Gao2; Ao, Zhuo3; Han, Dong3; Niu, Fenglan1; Wang, Shutao2 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:14页码:8318-8325 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c4nr01415d |
| 英文摘要 | Activated tumor-associated fibroblasts (TAFs) with abundant fibroblast activation protein (FAP) expression attract tremendous attention in tumor progression studies. In this work, we report a rapid 24 h FAP activation method for fibroblasts using silicon nanowires (SiNWs) as culture substrates instead of growth factors or chemokines. In contrast with cells cultured on flat silicon which rarely express FAP, SiNW cultivated cells exhibit FAP levels similar to those found in cancerous tissue. We demonstrated that activated cells grown on SiNWs maintain their viability and proliferation in a time-dependent manner. Moreover, environmental scanning electron microscopy (ESEM) and focused ion beam and scanning electron microscopy (FIB-SEM) analysis clearly revealed that activated cells on SiNWs adapt to the structure of their substrates by filling inter-wire cavities via filopodia in contrast to cells cultured on flat silicon which spread freely. We further illustrated that the expression of FAP was rarely detected in activated cells after being re-cultured in Petri dishes, suggesting that the unique structure of SiNWs may have a certain influence on FAP activation. |
| 语种 | 英语 |
| WOS记录号 | WOS:000338638900079 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49977]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Han, Dong |
| 作者单位 | 1.Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China 2.Chinese Acad Sci ICCAS, BNLMS, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China 3.Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ha, Qing,Yang, Gao,Ao, Zhuo,et al. Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays[J]. NANOSCALE,2014,6(14):8318-8325. |
| APA | Ha, Qing,Yang, Gao,Ao, Zhuo,Han, Dong,Niu, Fenglan,&Wang, Shutao.(2014).Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays.NANOSCALE,6(14),8318-8325. |
| MLA | Ha, Qing,et al."Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays".NANOSCALE 6.14(2014):8318-8325. |
入库方式： OAI收割
来源：化学研究所
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