A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality
文献类型：期刊论文
| 作者 | Zhou, Zhenpeng1; Li, Tian1; Huang, Hongduan1; Chen, Yang1; Liu, Feng1; Huang, Chengzhi2; Li, Na1 |
| 刊名 | CHEMICAL COMMUNICATIONS
 |
| 出版日期 | 2014-11-11 |
| 卷号 | 50期号:87页码:13373-13376 |
| ISSN号 | 1359-7345 |
| DOI | 10.1039/c4cc05554c |
| 英文摘要 | Silver-enhanced fluorescence was coupled with a bio-barcode assay to facilitate a dual amplification assay to demonstrate a non-enzymatic approach for simple and sensitive detection of DNA. In the assay design, magnetic nanoparticles seeded with silver nanoparticles were modified with the capture DNA, and silver nanoparticles were modified with the binding of ssDNA and the fluorescently labeled barcode dsDNA. Upon introduction of the target DNA, a sandwich structure was formed because of the hybridization reaction. By simple magnetic separation, silver-enhanced fluorescence of barcode DNAs could be readily measured without the need of a further step to liberate barcode DNAs from silver nanoparticles, endowing the method with simplicity and high sensitivity with a detection limit of 1 pM. |
| 语种 | 英语 |
| WOS记录号 | WOS:000343965700046 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50073]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Huang, Chengzhi |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem,Minist Educ, BNLMS,Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China 2.Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence & Real Time Anal, Educ Minist, Chongqing 400715, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhenpeng,Li, Tian,Huang, Hongduan,et al. A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality[J]. CHEMICAL COMMUNICATIONS,2014,50(87):13373-13376. |
| APA | Zhou, Zhenpeng.,Li, Tian.,Huang, Hongduan.,Chen, Yang.,Liu, Feng.,...&Li, Na.(2014).A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality.CHEMICAL COMMUNICATIONS,50(87),13373-13376. |
| MLA | Zhou, Zhenpeng,et al."A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality".CHEMICAL COMMUNICATIONS 50.87(2014):13373-13376. |
入库方式： OAI收割
来源：化学研究所
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