Single-Layer MnO2 Nanosheets Suppressed Fluorescence of 7-Hydroxycoumarin: Mechanistic Study and Application for Sensitive Sensing of Ascorbic Acid in Vivo
文献类型：期刊论文
| 作者 | Zhai, Wanying; Wang, Chunxia; Yu, Ping; Wang, Yuexiang; Mao, Lanqun |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-12-16 |
| 卷号 | 86期号:24页码:12206-12213 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac503215z |
| 英文摘要 | In this study, we systematically investigate the mechanism of single-layer MnO2 nanosheets suppressing fluorescence of 7-hydroxycoumarin and, based on this, demonstrate a new fluorescent method for in vivo sensing of ascorbic acid (AA) in rat brain. The mechanism for the fluorescence suppression is attributed to a combination of inner filter effect (IFE) and static quenching effect (SQE), which is different from those reported for the traditional two-dimensional nanosheets, and Forster resonant energy transfer (FRET) mechanism reported for MnO2 nanosheets. The combination of IFE and SQE leads to an exponential decay in fluorescence intensity of 7-hydroxycoumarin with increasing concentration of MnO2 nanosheets in solution. Such a property allows optimization of the concentration of MnO2 nanosheets in such a way that the addition of reductive analyte (e.g., AA) will to the greatest extent restore the MnO2 nanosheets-suppressed fluorescence of 7-hydroxycoumarin through the redox reaction between AA and MnO2 nanosheets. On the basis of this feature, we demonstrate a fluorescent method for in vivo sensing of AA in the cerebral systems with an improved sensitivity. Compared with the turn-on fluorescent method through first decreasing the fluorescence to the lowest level by adding concentrated MnO2 nanosheets, the method demonstrated here possesses a higher sensitivity, lower limit of detection, and wider linear range. Upon the use of ascorbate oxidase to achieve the selectivity for AA, the turn-on fluorescence method demonstrated here can be used for in vivo sensing of AA in a simple but reliable way. |
| 语种 | 英语 |
| WOS记录号 | WOS:000346683900043 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50127]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Mao, Lanqun |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Wanying,Wang, Chunxia,Yu, Ping,et al. Single-Layer MnO2 Nanosheets Suppressed Fluorescence of 7-Hydroxycoumarin: Mechanistic Study and Application for Sensitive Sensing of Ascorbic Acid in Vivo[J]. ANALYTICAL CHEMISTRY,2014,86(24):12206-12213. |
| APA | Zhai, Wanying,Wang, Chunxia,Yu, Ping,Wang, Yuexiang,&Mao, Lanqun.(2014).Single-Layer MnO2 Nanosheets Suppressed Fluorescence of 7-Hydroxycoumarin: Mechanistic Study and Application for Sensitive Sensing of Ascorbic Acid in Vivo.ANALYTICAL CHEMISTRY,86(24),12206-12213. |
| MLA | Zhai, Wanying,et al."Single-Layer MnO2 Nanosheets Suppressed Fluorescence of 7-Hydroxycoumarin: Mechanistic Study and Application for Sensitive Sensing of Ascorbic Acid in Vivo".ANALYTICAL CHEMISTRY 86.24(2014):12206-12213. |
入库方式： OAI收割
来源：化学研究所
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