Studies on the biological character of a new pH-sensitive doxorubicin prodrug with tumor targeting using a LC-MS/MS method
文献类型：期刊论文
| 作者 | Zhang, Ruixing1,2,4; Hu, Xiuli3; Song, Fengrui1,2; Liu, Zhiqiang1,2; Xie, Zhigang3; Jing, Xiabin3 |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:9页码:3159-3166 |
| ISSN号 | 1759-9660 |
| DOI | 10.1039/c4ay00065j |
| 英文摘要 | A pH-sensitive doxorubicin (Dox) prodrug was prepared, and its release in vitro and distribution in vivo were studied by using a high performance liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) method. The prodrug, mPEG-P(LA-co-DHP/Dox) (P(Dox)), was amphiphilic in nature, with a hydrophilic mPEG block and a hydrophobic P(LA-co-DHP/Dox) block. The Dox was connected to the carrier polymer chain via a hydrazine linkage. The in vitro release results showed that the release rate of Dox from P(Dox) in acidic medium (pH = 5.0) was dramatically higher than that under physiological conditions (pH = 7.4). The absorption of Dox from P(Dox) in HeLa cells was much faster than that of free Dox. The MTT cell viability study demonstrated the higher cytotoxicity of P(Dox) than free Dox at the same concentration. The Dox concentration from P(Dox) in the mouse liver was dramatically higher than those in other tissues. The above results showed that P(Dox), as a prodrug of Dox, greatly favors the bioavailability of doxorubicin and prolongs its circle time in vivo. |
| 语种 | 英语 |
| WOS记录号 | WOS:000334736500046 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50193]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Song, Fengrui |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, Chem Biol Lab, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun Ctr Mass Spectrometry, Changchun 130022, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ruixing,Hu, Xiuli,Song, Fengrui,et al. Studies on the biological character of a new pH-sensitive doxorubicin prodrug with tumor targeting using a LC-MS/MS method[J]. ANALYTICAL METHODS,2014,6(9):3159-3166. |
| APA | Zhang, Ruixing,Hu, Xiuli,Song, Fengrui,Liu, Zhiqiang,Xie, Zhigang,&Jing, Xiabin.(2014).Studies on the biological character of a new pH-sensitive doxorubicin prodrug with tumor targeting using a LC-MS/MS method.ANALYTICAL METHODS,6(9),3159-3166. |
| MLA | Zhang, Ruixing,et al."Studies on the biological character of a new pH-sensitive doxorubicin prodrug with tumor targeting using a LC-MS/MS method".ANALYTICAL METHODS 6.9(2014):3159-3166. |
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来源：化学研究所
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