Biocompatible click chemistry enabled compartment-specific pH measurement inside E-coli
文献类型：期刊论文
| 作者 | Yang, Maiyun1; Jalloh, Abubakar S.2; Wei, Wei3; Zhao, Jing3; Wu, Peng2; Chen, Peng R.1,4 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2014-09-01 |
| 卷号 | 5 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/ncomms5981 |
| 英文摘要 | Bioorthogonal reactions, especially the Cu(I)-catalysed azide-alkyne cycloaddition, have revolutionized our ability to label and manipulate biomolecules under living conditions. The cytotoxicity of Cu(I) ions, however, has hindered the application of this reaction in the internal space of living cells. By systematically surveying a panel of Cu(I)-stabilizing ligands in promoting protein labelling within the cytoplasm of Escherichia coli, we identify a highly efficient and biocompatible catalyst for intracellular modification of proteins by azide-alkyne cycloaddition. This reaction permits us to conjugate an environment-sensitive fluorophore site specifically onto HdeA, an acid-stress chaperone that adopts pH-dependent conformational changes, in both the periplasm and cytoplasm of E. coli. The resulting protein-fluorophore hybrid pH indicators enable compartment-specific pH measurement to determine the pH gradient across the E. coli cytoplasmic membrane. This construct also allows the measurement of E. coli transmembrane potential, and the determination of the proton motive force across its inner membrane under normal and acid-stress conditions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000342985300002 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50321]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Jing |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China 2.Yeshiva Univ, Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA 3.Nanjing Univ, Sch Life Sci, Inst Chem & BioMed Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China 4.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Maiyun,Jalloh, Abubakar S.,Wei, Wei,et al. Biocompatible click chemistry enabled compartment-specific pH measurement inside E-coli[J]. NATURE COMMUNICATIONS,2014,5. |
| APA | Yang, Maiyun,Jalloh, Abubakar S.,Wei, Wei,Zhao, Jing,Wu, Peng,&Chen, Peng R..(2014).Biocompatible click chemistry enabled compartment-specific pH measurement inside E-coli.NATURE COMMUNICATIONS,5. |
| MLA | Yang, Maiyun,et al."Biocompatible click chemistry enabled compartment-specific pH measurement inside E-coli".NATURE COMMUNICATIONS 5(2014). |
入库方式： OAI收割
来源：化学研究所
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