In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model
文献类型：期刊论文
| 作者 | Sun, Zhongchan1,2; Song, Xinxing2,3; Li, Xiujuan2; Su, Tao2; Qi, Shun4; Qiao, Ruirui5; Wang, Fu6; Huan, Yi4; Yang, Weidong7; Wang, Jing7 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:23页码:14343-14353 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c4nr03003f |
| 英文摘要 | miRNA-16 (miR16) plays an important role in modulating the drug resistance of SGC7901 cell lines to adriamycin (ADR). A variety of viral carriers have been designed for miRNA delivery. However, the safety concerns are currently perceived as hampering the clinical application of viral vector-based therapy. Herein a type of magnetic nanoparticles (MNPs) was designed and synthesized using poly(ethylene glycol) (PEG)-coated Fe3O4 nanoparticles as a miRNA delivery system for the purpose of reducing drug resistance of gastric cancer cells by enforcing miR16 expression in SGC7901/ADR cells. The MNPs with good biocompatibility were synthesized by thermal decomposition, and then conjugated with miRNA via electrostatic interaction producing miR16/MNPs. After co-culture with miR16/MNPs, ADR-induced apoptosis of SGC7901/ADR was examined by MTT and TUNEL. miR16/MNPs treatment significantly increased cell apoptosis in vitro. SGC7901/ADR(fluc) tumor-bearing nude mice under ADR therapy were treated with miR16/MNPs by tail vein injection for in vivo study. After intraperitoneal injection of ADR, tumor volume measurement and fluorescence imaging were performed to for the death of SGC7901/ADR cells in vivo. Results showed that miR16/MNPs were able to significantly suppress SGC7901/ADR tumor growth, probably through increasing SGC7901/ADR cells' sensitivity to ADR. Our results suggest the efficient delivery of miR16 by MNPs as a novel therapeutic strategy for drug resistant tumor treatment. |
| 语种 | 英语 |
| WOS记录号 | WOS:000344997600034 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50527]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cao, Feng |
| 作者单位 | 1.Chinese Peoples Liberat Army Gen Hosp, Dept Cardiol, Beijing 100853, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp, Dept Cardiol, Xian 710032, Peoples R China 3.285 Hosp, Dept Cardiol, Handan 056001, Peoples R China 4.Fourth Mil Med Univ, Xijing Hosp, Dept Radiol, Xian 710032, Peoples R China 5.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 6.Xidian Univ, Sch Life Sci & Technol, Life Sci Res Ctr, Xian 710071, Peoples R China 7.Fourth Mil Med Univ, Xijing Hosp, Dept Nucl Med, Xian 710032, Peoples R China 8.Fourth Mil Med Univ, Xijing Hosp, Dept Digest Dis, Xian 710032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Zhongchan,Song, Xinxing,Li, Xiujuan,et al. In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model[J]. NANOSCALE,2014,6(23):14343-14353. |
| APA | Sun, Zhongchan.,Song, Xinxing.,Li, Xiujuan.,Su, Tao.,Qi, Shun.,...&Cao, Feng.(2014).In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model.NANOSCALE,6(23),14343-14353. |
| MLA | Sun, Zhongchan,et al."In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model".NANOSCALE 6.23(2014):14343-14353. |
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来源：化学研究所
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