Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase
文献类型：期刊论文
| 作者 | Chen, Tengfei1,2,3; Gu, Jiangyong2; Wang, Hao2; Yuan, Gu2; Chen, Lirong2; Xu, Xiaojie2; Xiao, Wei1,3 |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 77期号:13-14页码:893-899 |
| ISSN号 | 0009-5893 |
| 关键词 | Liquid Chromatography Molecularly Imprinted Polymer Semi-preparative Separation Emodin Rheum PaL.atum L. |
| DOI | 10.1007/s10337-014-2691-z |
| 英文摘要 | In this work, a core-shell molecularly imprinted polymer (MIP) was synthesized through sol-gel coating procedure by using silica beads, emodin, 3-aminopropyltriethoxysilane, tetraethoxysilane and tetrahydrofuran as supporting matrix, template, functional monomer, cross-linker and solvent, respectively. The selective recognition property for emodin and its analogues (physcion and aloe emodin) of the resultant MIP was evaluated in a chromatographic column for high-performance liquid chromatography (HPLC). The retention time and imprinted factor of MIP column for emodin, physcion and aloe emodin were 5.11, 0.63, 0.69 min and 4.69, 0.75, 1.38, respectively. It showed that the MIP beads had a good binding ability for emodin. Finally, one-step separation of emodin from alcohol extract of Rheum palmatum L. at semi-preparative scale was achieved and 380 mg of emodin was collected in 5 days. The product was characterized by mass spectrometry and HPLC and its purity was 95 %. |
| 语种 | 英语 |
| 出版者 | SPRINGER HEIDELBERG |
| WOS记录号 | WOS:000339005800004 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50761]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xiao, Wei |
| 作者单位 | 1.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100102, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Rare Earth Mat Chem & Applicat, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Jiangsu Kanion Pharmaceut Co Ltd, State Key Lab New Tech Chinese Med Pharmaceut Pro, Lianyungang 222001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tengfei,Gu, Jiangyong,Wang, Hao,et al. Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase[J]. CHROMATOGRAPHIA,2014,77(13-14):893-899. |
| APA | Chen, Tengfei.,Gu, Jiangyong.,Wang, Hao.,Yuan, Gu.,Chen, Lirong.,...&Xiao, Wei.(2014).Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase.CHROMATOGRAPHIA,77(13-14),893-899. |
| MLA | Chen, Tengfei,et al."Semi-Preparative Scale Separation of Emodin from Plant Extract by Using Molecularly Imprinted Polymer as Stationary Phase".CHROMATOGRAPHIA 77.13-14(2014):893-899. |
入库方式： OAI收割
来源：化学研究所
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