Enantioseparation of dansyl amino acids and dipeptides by chiral ligand exchange capillary electrophoresis based on Zn(II)-L-hydroxyproline complexes coordinating with gamma-cyclodextrins
文献类型：期刊论文
| 作者 | Mu, Xiaoyu1,2; Qi, Li1; Qiao, Juan1; Yang, Xinzheng3; Ma, Huimin1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2014-10-10 |
| 卷号 | 846页码:68-74 |
| 关键词 | Chiral Ligand Exchange Capillary Electrophoresis L-hydroxyproline Amino Acid Dipeptide Gamma-cyclodextrin |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2014.07.022 |
| 英文摘要 | A chiral ligand exchange capillary electrophoresis (CLE-CE) method using Zn(II) as the central ion and L-4-hydroxyproline as the chiral ligand coordinating with gamma-cyclodextrin (gamma-CD) was developed for the enantioseparation of amino acids (AAs) and dipeptides. The effects of various separation parameters, including the pH of the running buffer, the ratio of Zn(II) to L-4-hydroxyproline, the concentration of complexes and cyclodextrins (CDs) were systematically investigated. After optimization, it has been found that eight pairs of labeled AAs and six pairs of labeled dipeptides could be baseline-separated with a running electrolyte of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM Zn(II), 6.0 mM L-hydroxyproline and 4.0 mM gamma-CD at pH 8.2. The quantitation of AAs and dipeptides was conducted and good linearity (r(2) >= 0.997) and favorable repeatability (RSD <= 3.6%) were obtained. Furthermore, the proposed method was applied in determining the enantiomeric purity of AAs and dipeptides. Meanwhile, the possible enantiorecognition mechanism based on the synergistic effect of chiral metal complexes and gamma-CD was explored and discussed briefly. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000342167200007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50885]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Xiaoyu,Qi, Li,Qiao, Juan,et al. Enantioseparation of dansyl amino acids and dipeptides by chiral ligand exchange capillary electrophoresis based on Zn(II)-L-hydroxyproline complexes coordinating with gamma-cyclodextrins[J]. ANALYTICA CHIMICA ACTA,2014,846:68-74. |
| APA | Mu, Xiaoyu,Qi, Li,Qiao, Juan,Yang, Xinzheng,&Ma, Huimin.(2014).Enantioseparation of dansyl amino acids and dipeptides by chiral ligand exchange capillary electrophoresis based on Zn(II)-L-hydroxyproline complexes coordinating with gamma-cyclodextrins.ANALYTICA CHIMICA ACTA,846,68-74. |
| MLA | Mu, Xiaoyu,et al."Enantioseparation of dansyl amino acids and dipeptides by chiral ligand exchange capillary electrophoresis based on Zn(II)-L-hydroxyproline complexes coordinating with gamma-cyclodextrins".ANALYTICA CHIMICA ACTA 846(2014):68-74. |
入库方式： OAI收割
来源：化学研究所
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