Continuous and Simultaneous Electrochemical Measurements of Glucose, Lactate, and Ascorbate in Rat Brain Following Brain Ischemia
文献类型：期刊论文
| 作者 | Lin, Yuqing1,2; Yu, Ping1; Hao, Jie1; Wang, Yuexiang1; Ohsaka, Takeo3; Mao, Lanqun1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-04-15 |
| 卷号 | 86期号:8页码:3895-3901 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac4042087 |
| 英文摘要 | Developing new tools and technologies to enable recording the dynamic changes of multiple neurochemicals is the essence of better understanding of the molecular basis of brain functions. This study demonstrates a microfluidic chip-based online electrochemical system (OECS) for in vivo continuous and simultaneous monitoring of glucose, lactate, and ascorbate in rat brain. To fabricate the, microfluidic chip-based detecting system, a microfluidic chip.. with patterned channel is developed into an electrochemical flow cell by incorporating the chip with three surface-modified indium-tin oxide (ITO) electrodes as working electrodes, a Ag/AgCl wire as reference electrode, and a stainless steel tube as counter electrode. Selective detection of ascorbate is achieved by the use of single-walled carbon nanotubes (SWNTs) to largely facilitate the electrochemical oxidation of ascorbate, while a dehydrogenase-based biosensing mechanism with methylene green (MG) adsorbed onto SWNTs as an electrocatalyst for the oxidation of dihydronicotiamide adenine dinucleotide (NADH) is employed for biosensing of glucose and lactate. To avoid the crosstalk among three sensors, the sensor alignment is carefully designed with the SWNT-modified electrode in the upstream channel and paralleled glucose and lactate biosensors in the downstream channels. With the microfluidic chip-based electrochemical flow cell as the detector, an OECS is successfully established by directly integrating the microfluidic chip-based electrochemical flow cell with in vivo microdialysis. The OECS exhibits a good linear response toward glucose, lactate, and ascorbate with less crosstalk. This property, along with the high stability and selectivity, enables the OECS for continuously monitoring three species in rat brain following brain ischemia. |
| 语种 | 英语 |
| WOS记录号 | WOS:000334658000033 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50949]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Mao, Lanqun |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China 3.Tokyo Inst Technol, Dept Elect Chem, Interdisciplinary Grad Sch Sci & Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan |
| 推荐引用方式 GB/T 7714 | Lin, Yuqing,Yu, Ping,Hao, Jie,et al. Continuous and Simultaneous Electrochemical Measurements of Glucose, Lactate, and Ascorbate in Rat Brain Following Brain Ischemia[J]. ANALYTICAL CHEMISTRY,2014,86(8):3895-3901. |
| APA | Lin, Yuqing,Yu, Ping,Hao, Jie,Wang, Yuexiang,Ohsaka, Takeo,&Mao, Lanqun.(2014).Continuous and Simultaneous Electrochemical Measurements of Glucose, Lactate, and Ascorbate in Rat Brain Following Brain Ischemia.ANALYTICAL CHEMISTRY,86(8),3895-3901. |
| MLA | Lin, Yuqing,et al."Continuous and Simultaneous Electrochemical Measurements of Glucose, Lactate, and Ascorbate in Rat Brain Following Brain Ischemia".ANALYTICAL CHEMISTRY 86.8(2014):3895-3901. |
入库方式： OAI收割
来源：化学研究所
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