Identification of FkpA as a Key Quality Control Factor for the Biogenesis of Outer Membrane Proteins under Heat Shock Conditions
文献类型：期刊论文
| 作者 | Ge, Xi1; Lyu, Zhi-Xin2; Liu, Yang1; Wang, Rui1; Zhao, Xin Sheng2; Fu, Xinmiao1; Chang, Zengyi1,3 |
| 刊名 | JOURNAL OF BACTERIOLOGY
 |
| 出版日期 | 2014-02-01 |
| 卷号 | 196期号:3页码:672-680 |
| ISSN号 | 0021-9193 |
| DOI | 10.1128/JB.01069-13 |
| 英文摘要 | The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable beta- barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the Delta surA Delta skp double-deletion strain of E. coli, although lethal and defective in the biogenesis of OMPs at the normal growth temperature, is viable and effective at the heat shock temperature. We identified FkpA as the multicopy suppressor for the lethal phenotype of the Delta surA Delta skp strain. We also demonstrated that the deletion of fkpA from the Delta surA cells resulted in only a mild decrease in the levels of folded OMPs at the normal temperature but a severe decrease as well as lethality at the heat shock temperature, whereas the deletion of fkpA from the Delta skp cells had no detectable effect on OMP biogenesis at either temperature. These results strongly suggest a functional redundancy between FkpA and SurA for OMP biogenesis under heat shock stress conditions. Mechanistically, we found that FkpA becomes a more efficient chaperone for OMPs under the heat shock condition, with increases in both binding rate and affinity. In light of these observations and earlier reports, we propose a temperature-responsive OMP biogenesis mechanism in which the degrees of functional importance of the three chaperones are such that SurA> Skp> FkpA at the normal temperature but FkpA >= SurA> Skp at the heat shock temperature. |
| 语种 | 英语 |
| WOS记录号 | WOS:000332625000017 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50957]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chang, Zengyi |
| 作者单位 | 1.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Biodynam Opt Imaging Ctr,Dept Chem Biol, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China 3.Peking Univ, Ctr Prot Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Xi,Lyu, Zhi-Xin,Liu, Yang,et al. Identification of FkpA as a Key Quality Control Factor for the Biogenesis of Outer Membrane Proteins under Heat Shock Conditions[J]. JOURNAL OF BACTERIOLOGY,2014,196(3):672-680. |
| APA | Ge, Xi.,Lyu, Zhi-Xin.,Liu, Yang.,Wang, Rui.,Zhao, Xin Sheng.,...&Chang, Zengyi.(2014).Identification of FkpA as a Key Quality Control Factor for the Biogenesis of Outer Membrane Proteins under Heat Shock Conditions.JOURNAL OF BACTERIOLOGY,196(3),672-680. |
| MLA | Ge, Xi,et al."Identification of FkpA as a Key Quality Control Factor for the Biogenesis of Outer Membrane Proteins under Heat Shock Conditions".JOURNAL OF BACTERIOLOGY 196.3(2014):672-680. |
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来源：化学研究所
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