Detection of glucose via enzyme-coupling reaction based on a DT-diaphorase fluorescence probe
文献类型：期刊论文
| 作者 | Gao, Xinghui; Li, Xiaohua; Wan, Qiongqiong; Li, Zhao; Ma, Huimin |
| 刊名 | TALANTA
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 120页码:456-461 |
| 关键词 | Enzyme-coupling Reaction Fluorescence Probe Resorufin Glucose |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2013.12.032 |
| 英文摘要 | Enzyme-coupling reactions play an important role in the assay of analytes. In this manuscript, we developed a new fluorescent probe for the detection of glucose through the enzyme-coupling reaction of DT-diaphorase (DTD). The probe was synthesized through a mild and simple synthetic procedure, and showed good fluorescence response to DID. The reactions for the detection of glucose proceed as follows: glucose dehydrogenase oxidizes glucose to gluconolactone with NAD(+) as the electron acceptor to yield NADH, and NADH can be utilized by DID to further react with the probe releasing resorufin. As a result of these tandem reactions, fluorescence off-on response will occur. The method showed high selectivity for glucose with a detection limit of 0.2 mu M which may provide a potential way for fluorescence detection of glucose through enzyme-coupling reactions. Furthermore, the applicability of the method has been demonstrated by detecting glucose in human urine samples. (C) 2013 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000331917700063 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50977]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Xiaohua |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xinghui,Li, Xiaohua,Wan, Qiongqiong,et al. Detection of glucose via enzyme-coupling reaction based on a DT-diaphorase fluorescence probe[J]. TALANTA,2014,120:456-461. |
| APA | Gao, Xinghui,Li, Xiaohua,Wan, Qiongqiong,Li, Zhao,&Ma, Huimin.(2014).Detection of glucose via enzyme-coupling reaction based on a DT-diaphorase fluorescence probe.TALANTA,120,456-461. |
| MLA | Gao, Xinghui,et al."Detection of glucose via enzyme-coupling reaction based on a DT-diaphorase fluorescence probe".TALANTA 120(2014):456-461. |
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