New Monomethine Cyanine Dye and Its Interaction with Different DNA Forms
文献类型：期刊论文
| 作者 | Wu Shangrong1,2; Jin Bing2; Zhang Nan2; Liu Ying2; Liu Xiangjun2; Li Songqing1; Shangguan Dihua2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2014-10-10 |
| 卷号 | 35期号:10页码:2085-2092 |
| 关键词 | Cyanine Dye Dna G-quadruplex Fluorescent Probe Nucleic Acid Ligand |
| ISSN号 | 0251-0790 |
| DOI | 10.7503/cjcu20140562 |
| 英文摘要 | Cyanine dyes have been widely used as fluorescent probes for nucleic acids. A well-known monomethine cyanine dye, thiazole orange(TO), has been reported to bind various forms of nucleic acids, such as RNA, duplex DNA, triplex DNA and especially G-quadruplex DNA. As a nucleic acid light-up probe, TO has been used in a variety of applications. The binding modes between TO and G-quadruplex DNA are complex, may include end-stacking, groove binding and external stacking, the predominant binding mode is reported to be end-stacking. In order to further understand the interaction of monomethine cyanines with different nucleic acid forms and develop new probes, we synthesized a new analogue of TO, 1,2-dimethy1-6-{[3-methylbenzo[d]thiazol-2(3H)-ylidene]methyl} pyridine-1-ium (MTP) and investigated its interaction with different DNA forms. The interaction of MTP with c-myc (parallel G-quadruplex), 22AGK(+) (mixed-type G-quadruplex), ss/dsDNA(single/double-stranded DNA) caused notable red-shift and hypochromicity of the absorption spectrum of MTP. MTP exhibited almost no fluorescence in aqueous buffer condition. However, the interaction of MTP with DNA resulted in great enhancement of MTP fluorescence, approximately 130-180-fold for c-myc or 22AGK(+), 40-60-fold for single/double-stranded (ss/ds) DNA and 15-25-fold for TBA and 22AGNa(+)(antiparallel G-quadruplexes). The apparent dissociation constants (K-d) of MTP and different DNA were in the range of 4.0-17 mu mol/L. The fluorescence enhancement was corrected with the binding affinity of MTP and different DNA forms. These results suggest that MTP can be used as a fluorescent probe to distinguish different forms of nucleic acids. The binding stoichiometry showed that two molecules of MTP bound to one molecule of c-myc or 22AGK(+). The induced CD spectroscopy and G-quadruplex/hemin peroxidase inhibition experiment suggested that the first MTP bound to c-myc through the groove binding model and the second MTP bound to c-myc through the end-stacking model. Compared with TO, MTP has a smaller size; it showed different absorption spectral changes upon binding to DNA and exhibited different fluorescence responses to 22AGK(+) and 22AGNa(+), which may suggest the different binding modes of TO and MTP to G-quadruplexes. These results provide important information for the design of the ligands and probes for nucleic acids. |
| 语种 | 英语 |
| WOS记录号 | WOS:000344833200006 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50983]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li Songqing |
| 作者单位 | 1.Xiangtan Univ, Colloge Chem, Xiangtan 411100, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu Shangrong,Jin Bing,Zhang Nan,et al. New Monomethine Cyanine Dye and Its Interaction with Different DNA Forms[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2014,35(10):2085-2092. |
| APA | Wu Shangrong.,Jin Bing.,Zhang Nan.,Liu Ying.,Liu Xiangjun.,...&Shangguan Dihua.(2014).New Monomethine Cyanine Dye and Its Interaction with Different DNA Forms.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,35(10),2085-2092. |
| MLA | Wu Shangrong,et al."New Monomethine Cyanine Dye and Its Interaction with Different DNA Forms".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 35.10(2014):2085-2092. |
入库方式： OAI收割
来源：化学研究所
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