Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells
文献类型：期刊论文
| 作者 | Li, Wan-Ming1,2; Bing, Tao3; Wei, Jia-Yi1,2; Chen, Zhe-Zhou1,2; Shangguan, Di-Hua3; Fang, Jin1,2 |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 35期号:25页码:6998-7007 |
| 关键词 | Cell-selex Aptamer Metastatic Colorectal Cancer Drug Delivery Molecular Imaging Quantum Dots |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2014.04.112 |
| 英文摘要 | The development of diagnostic/therapeutic strategies against metastasis-related molecular targets is critical for improving the survival rate of cancer patients. Subtractive Cell-SELEX was performed using highly metastatic colorectal cancer (CRC) LoVo cells and non-metastatic HCT-8 cells as the target and negative cells, respectively, for the selection of metastatic-specific aptamers. This process generated seven aptamers that displayed highly specific binding to the target cells with K(d)s in the nanomolar range. Based on the distinct chemical/biological properties of their individual cell surface targets, the aptamers were separately functionalized: the receptor-targeting aptamer W14 was used as a carrier for doxorubicin, resulting in the specific delivery of the drug to the target cells and a significant reduction of its cytotoxicity to non-target cells, and the non-receptor-binding aptamer W3 was used as a molecular probe conjugated to quantum dots for the targeted imaging of metastatic cancer cell lines, spontaneous lung metastasis murine tissue, and metastatic CRC patient tissues. In addition, these aptamers can be used in combination due to their lack of detectable mutual-binding interference. The study demonstrates that a panel of aptamers that recognize distinct features of target molecules can be obtained through single Cell-SELEX selection, and the selected aptamers may be individually functionalized for specific applications and/or utilized in combination. (C) 2014 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000338386800031 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/50995]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Jin |
| 作者单位 | 1.China Med Univ, Dept Cell Biol, Key Lab Cell Biol, Minist Publ Hlth, Shenyang 110001, Peoples R China 2.China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wan-Ming,Bing, Tao,Wei, Jia-Yi,et al. Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells[J]. BIOMATERIALS,2014,35(25):6998-7007. |
| APA | Li, Wan-Ming,Bing, Tao,Wei, Jia-Yi,Chen, Zhe-Zhou,Shangguan, Di-Hua,&Fang, Jin.(2014).Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells.BIOMATERIALS,35(25),6998-7007. |
| MLA | Li, Wan-Ming,et al."Cell-SELEX-based selection of aptamers that recognize distinct targets on metastatic colorectal cancer cells".BIOMATERIALS 35.25(2014):6998-7007. |
入库方式： OAI收割
来源：化学研究所
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