Identification and discrimination of binding sites of an organoruthenium anticancer complex to single-stranded oligonucleotides by mass spectrometry
文献类型：期刊论文
| 作者 | Liu, Suyan; Wu, Kui; Zheng, Wei; Zhao, Yao; Luo, Qun; Xiong, Shaoxiang; Wang, Fuyi |
| 刊名 | ANALYST
 |
| 出版日期 | 2014-09-21 |
| 卷号 | 139期号:18页码:4491-4496 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c4an00807c |
| 英文摘要 | We here report the identification of the binding sites of an organometallic ruthenium anticancer complex [(eta(6)-biphenyl)Ru(en)Cl](+) (1) to single-stranded oligodeoxynucleotides (ODNs) 5'-CCCA(4)G(5)C(6)CC-3' (I) and 5'-CCC(3)G(4)A(5)CCC-3' (II) by mass spectrometry. The MS analysis of exonuclease ladders demonstrated that the 5'-exonuclease bovine spleen phosphodiesterase digestion of I and II mono-ruthenated by complex 1 was arrested solely at A(4) and partially at C-3 and G(4), respectively, and that the 3'-exonuclease snake venom phosphodiesterase digestion of the ruthenated ODNs was arrested solely at G(5) and G(4), respectively, due to the ruthenation. These results did not allow unambiguous identification of ruthenation sites on the metallated ODNs. In contrast, tandem mass spectrometry analysis with CID fragmentation of the mono-ruthenated ODNs provided sequential and complementary [a(i) - B]/wi fragments, leading to unambiguous identification of G(5) in I and G(4) in II as the ruthenation sites on the ODN adducts, which is in line with the high selectivity of the complex towards guanine base as reported previously. These findings suggest that caution should be raised with regard to the identification of the binding sites of metal complexes, in particular complexes with bulky ligands, like biphenyl in complex 1, to DNA by MS analysis of exonuclease ladders of the metallated adducts, because the bulky ligands may adopt such an orientation that they block the exonuclease cleavage of the 5' -or 3'-side phosphodiester bonds adjacent to the binding sites, leading to digestion stalling at the nucleotides before the binding sites. |
| 语种 | 英语 |
| WOS记录号 | WOS:000340755000011 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51013]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wu, Kui |
| 作者单位 | Chinese Acad Sci, Beijing Ctr Mass Spectrometry, Inst Chem, CAS Key Lab Analyt Chem Living Biosyst,Beijing Na, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Suyan,Wu, Kui,Zheng, Wei,et al. Identification and discrimination of binding sites of an organoruthenium anticancer complex to single-stranded oligonucleotides by mass spectrometry[J]. ANALYST,2014,139(18):4491-4496. |
| APA | Liu, Suyan.,Wu, Kui.,Zheng, Wei.,Zhao, Yao.,Luo, Qun.,...&Wang, Fuyi.(2014).Identification and discrimination of binding sites of an organoruthenium anticancer complex to single-stranded oligonucleotides by mass spectrometry.ANALYST,139(18),4491-4496. |
| MLA | Liu, Suyan,et al."Identification and discrimination of binding sites of an organoruthenium anticancer complex to single-stranded oligonucleotides by mass spectrometry".ANALYST 139.18(2014):4491-4496. |
入库方式： OAI收割
来源：化学研究所
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