Intracellular temperature sensing by a ratiometric fluorescent polymer thermometer
文献类型：期刊论文
| 作者 | Qiao, Juan1; Chen, Chuanfang2; Qi, Li1; Liu, Meirong1; Dong, Ping4,5; Jiang, Qin1; Yang, Xinzheng3; Mu, Xiaoyu1; Mao, Lanqun1 |
| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
 |
| 出版日期 | 2014 |
| 卷号 | 2期号:43页码:7544-7550 |
| ISSN号 | 2050-750X |
| DOI | 10.1039/c4tb01154f |
| 英文摘要 | Intracellular temperature imaging could help to understand diverse biological reactions and functions in living cells. Herein, we report a nanothermometer based on ratiometric fluorescent polymers (RFPs) synthesized by the living/controlled reversible addition-fragmentation transfer polymerization method for intracellular temperature sensing. The thermometer was composed of a thermo sensitive polymer, a polarity sensitive fluorescent dye and a thermo insensitive fluorescent dye. With the increasing temperature, the fluorescent intensity of RFPs increased because of the fluorescent intensity enhancement of the polarity sensitive fluorescent dye induced by the self assembling of the thermo sensitive polymer. The prepared RFPs exhibited a fluorescence "turn-on" response at higher temperature. We further investigated the simultaneous temperature sensing and the ratiometric imaging of temperature variations associated with biological processes in living cells using this novel ratiometric probe. The polymer based ratiometric fluorescent thermometer can be used to precisely measure the temperature in living cells, and shows great potential in spatio-temporal temperature sensing down to the nanoscale within biological systems. |
| 语种 | 英语 |
| WOS记录号 | WOS:000343992700008 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51055]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qiao, Juan |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Inst Elect Engn, Beijing Key Lab Bioelectromagnetism, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 4.Chinese Acad Med Sci, Res Ctr Plast Surg Hosp, Beijing 100730, Peoples R China 5.Peking Union Med Coll, Beijing 100021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Juan,Chen, Chuanfang,Qi, Li,et al. Intracellular temperature sensing by a ratiometric fluorescent polymer thermometer[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(43):7544-7550. |
| APA | Qiao, Juan.,Chen, Chuanfang.,Qi, Li.,Liu, Meirong.,Dong, Ping.,...&Mao, Lanqun.(2014).Intracellular temperature sensing by a ratiometric fluorescent polymer thermometer.JOURNAL OF MATERIALS CHEMISTRY B,2(43),7544-7550. |
| MLA | Qiao, Juan,et al."Intracellular temperature sensing by a ratiometric fluorescent polymer thermometer".JOURNAL OF MATERIALS CHEMISTRY B 2.43(2014):7544-7550. |
入库方式： OAI收割
来源：化学研究所
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