Long-term kinetics of DNA interacting with polycations
文献类型：期刊论文
| 作者 | Zheng, Cui1; Niu, Lin1; Pan, Wei1; Zhou, Jihan1; Lv, Hua2; Cheng, Jianjun2; Liang, Dehai1 |
| 刊名 | POLYMER
 |
| 出版日期 | 2014-05-13 |
| 卷号 | 55期号:10页码:2464-2471 |
| ISSN号 | 0032-3861 |
| 关键词 | Polyelectrolyte Dna Complex Kinetics |
| DOI | 10.1016/j.polymer.2014.03.038 |
| 英文摘要 | DNA complex has been widely used as non-viral vectors for the delivery of genes or siRNA. Owing to the strong and long-ranged electrostatic interaction, the structure and property of the DNA complex should evolve with time in a long-term. To test this hypothesis, we choose 2000 bp double-stranded DNA and 21 bp oligonucleotide as the model molecules, and comparatively studied their complexation with narrowly-distributed poly-L-lysine (PLL150) by time-resolved laser light scattering. In the time range of about one week, the complexation of both DNA samples underwent three stages: formation of preliminary complex, further aggregation, followed by precipitation. The aggregation and precipitation rate of the complex formed by oligonucleotide was much faster than that of the complex formed by 2000 bp dsDNA. After precipitation, the amount of the longer chain polyelectrolyte, as determined by UV and fluorescence, was about twice that of the short chain polyelectrolyte in the sediment. The precipitates were far from being fully neutralized. Moreover, the component ratio in the sediment was independent of the mixing charge molar ratio. A rational complex mechanism was proposed on the basis of these findings. During complexation, the relaxation of polyelectrolyte inside the complex and the exchange of polyelectrolyte between complex determined the aggregation and precipitation rate. The competition of the two kinetic processes governed the structure and property of the complex in the solution and in the sediment. (c) 2014 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000335936500022 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51089]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liang, Dehai |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Polymer Chem & Phys, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China 2.Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA |
| 推荐引用方式 GB/T 7714 | Zheng, Cui,Niu, Lin,Pan, Wei,et al. Long-term kinetics of DNA interacting with polycations[J]. POLYMER,2014,55(10):2464-2471. |
| APA | Zheng, Cui.,Niu, Lin.,Pan, Wei.,Zhou, Jihan.,Lv, Hua.,...&Liang, Dehai.(2014).Long-term kinetics of DNA interacting with polycations.POLYMER,55(10),2464-2471. |
| MLA | Zheng, Cui,et al."Long-term kinetics of DNA interacting with polycations".POLYMER 55.10(2014):2464-2471. |
入库方式： OAI收割
来源：化学研究所
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