Evaluation of serum phosphopeptides as potential cancer biomarkers by mass spectrometric absolute quantification
文献类型：期刊论文
| 作者 | Zhai, Guijin1; Wu, Xiaoyan2; Luo, Qun1; Wu, Kui1; Zhao, Yao1; Liu, Jianan1; Xiong, Shaoxiang1; Feng, Yu-Qi3; Yang, Liping2; Wang, Fuyi1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 125页码:411-417 |
| 关键词 | Absolute Quantification Cancer Biomarker Mass Spectrometry Serum Phosphopeptides Enrichment |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2014.03.025 |
| 英文摘要 | Mass spectrometric quantification of phosphopeptides is a challenge due to the ion suppression effect of highly abundant non-phosphorylated peptides in complex samples such as serum. Several strategies for relative quantification of serum phosphopeptides based on MS have been developed, but the power of relative quantities was limited when making direct comparisons between two groups of samples or acting as a clinical examination index. Herein, we describe an MS absolute quantification strategy combined with Titania Coated Magnetic Hollow Mesoporous Silica Microspheres (TiO2/MHMSM) enrichment and stable isotopic acetyl labeling for phosphopeptides in human serum. Four endogenous serum phosphopeptides generated by degradation of fibrinogen were identified by LC-ESI-MS/MS following TiO2/MHMSM enrichment. The ESI-MS signal intensity ratios of the four phosphopeptide standards labeled with N-acetoxy-H-3-succinimide (H3-NAS) and N-acetoxy-D-3-succinimide (D3-NAS), following TiO2/MHMSM capture are linearly correlated with the molar ratios of the "light" to "heavy" phosphopeptides over the range of 0.1-4 with an r(2) of up to 0.998 and a slope of close to 1. The recovery of the four phosphopeptides spiked at low, medium and high levels in human sera were 98.4-111.9% with RSDs ranging 2.0-10.1%. The absolute quantification of the phosphopeptides in serum samples of 20 healthy persons and 20 gastric cancer patients by the developed method demonstrated that 3 out of the 4 phosphopeptides showed remarkable variation in serum level between healthy and cancer groups, and the phosphopeptide DpSGEGDFLAEGGGVR is significantly down-regulated in the serum of patients, being a potential biomarker for gastric cancer diagnosis. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000336872200059 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51119]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Liping |
| 作者单位 | 1.Chinese Acad Sci, Beijing Ctr Mass Spectrometry, Inst Chem, CAS Key Lab Analyt Chem Living Biosyst,Beijing Na, Beijing 100190, Peoples R China 2.Nantong Univ, Tumor Hosp, Canc Res Ctr, Nantong 226361, Jiangsu, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Guijin,Wu, Xiaoyan,Luo, Qun,et al. Evaluation of serum phosphopeptides as potential cancer biomarkers by mass spectrometric absolute quantification[J]. TALANTA,2014,125:411-417. |
| APA | Zhai, Guijin.,Wu, Xiaoyan.,Luo, Qun.,Wu, Kui.,Zhao, Yao.,...&Wang, Fuyi.(2014).Evaluation of serum phosphopeptides as potential cancer biomarkers by mass spectrometric absolute quantification.TALANTA,125,411-417. |
| MLA | Zhai, Guijin,et al."Evaluation of serum phosphopeptides as potential cancer biomarkers by mass spectrometric absolute quantification".TALANTA 125(2014):411-417. |
入库方式： OAI收割
来源：化学研究所
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