Visualization of the post-Golgi vesicle-mediated transportation of TGF-beta receptor II by quasi-TIRFM
文献类型：期刊论文
| 作者 | Luo, Wangxi1; Xia, Tie1; Xu, Li1; Chen, Ye-Guang2; Fang, Xiaohong1 |
| 刊名 | JOURNAL OF BIOPHOTONICS
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 7期号:10页码:788-798 |
| 关键词 | Quasi-tirfm Live-cell Imaging Post-golgi Transport Transforming Growth Factor Beta Receptor Ii Green Fluorescent Protein |
| ISSN号 | 1864-063X |
| DOI | 10.1002/jbio.201300020 |
| 英文摘要 | Transforming growth factor beta receptor II (T beta RII) is synthesized in the cytoplasm and then transported to the plasma membrane of cells to fulfil its signalling duty. Here, we applied live-cell fluorescence imaging techniques, in particular quasi-total internal reflection fluorescence microscopy, to imaging fluorescent protein-tagged TbRII and monitoring its secretion process. We observed punctuate-like T beta RII-containing post-Golgi vesicles formed in MCF7 cells. Single-particle tracking showed that these vesicles travelled along the microtubules at an average speed of 0.51 mu m/s. When stimulated by TGF-beta ligand, these receptor-containing vesicles intended to move towards the plasma membrane. We also identified several factors that could inhibit the formation of such post-Golgi vesicles. Although the inhibitory mechanisms still remain unknown, the observed characteristics of T beta RII-containing vesicles provide new information on intracellular TbRII transportation. It also renders T beta RII |
| 语种 | 英语 |
| WOS记录号 | WOS:000342847200004 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51227]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Wangxi,Xia, Tie,Xu, Li,et al. Visualization of the post-Golgi vesicle-mediated transportation of TGF-beta receptor II by quasi-TIRFM[J]. JOURNAL OF BIOPHOTONICS,2014,7(10):788-798. |
| APA | Luo, Wangxi,Xia, Tie,Xu, Li,Chen, Ye-Guang,&Fang, Xiaohong.(2014).Visualization of the post-Golgi vesicle-mediated transportation of TGF-beta receptor II by quasi-TIRFM.JOURNAL OF BIOPHOTONICS,7(10),788-798. |
| MLA | Luo, Wangxi,et al."Visualization of the post-Golgi vesicle-mediated transportation of TGF-beta receptor II by quasi-TIRFM".JOURNAL OF BIOPHOTONICS 7.10(2014):788-798. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。