中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection

文献类型:期刊论文

作者Nie, Ji; Zhang, De-Wen; Tie, Cai; Zhou, Ying-Lin; Zhang, Xin-Xiang
刊名BIOSENSORS & BIOELECTRONICS
出版日期2014-06-15
卷号56页码:237-242
关键词G-quadruplex Dna Detection Isothermal Exponential Amplification Label-free Colorimetry
ISSN号0956-5663
DOI10.1016/j.bios.2014.01.032
英文摘要A novel G-quadruplex based two-stage isothermal exponential amplification reaction (GQ-EXPAR) was developed for label-free DNA calorimetric detection in this work. The exponential amplified trigger DNA in the first stage can convert into G-quadruplex sequence EAD2 by a linear amplification circuit in the second stage. Created EAD2 can form G-quadruplex/hemin DNAzyme to act as a direct signal readout element. The GQ-EXPAR combines the exponential amplification of DNA sequence and the peroxidase-mimicking DNAzyme induced signal amplification, which achieves tandem dual-amplification. Taking advantages of isothermal incubation, this label-free homogeneous assay obviates the need of thermal cycling. As no complex synthesis or extra downstream operation is needed, the whole easy handling procedure can be finished in no more than 1 h. This assay allows the sensing of the model DNA with the limit of detection to be 2.5 pM. Moreover, it demonstrates good discrimination of mismatched sequences. The strategy has also been successfully implemented to sensitively detect Tay Sachs genetic disorder mutant. (C) 2014 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000333781000037
出版者ELSEVIER ADVANCED TECHNOLOGY
源URL[http://ir.iccas.ac.cn/handle/121111/51281]  
专题中国科学院化学研究所
通讯作者Zhang, Xin-Xiang
作者单位Peking Univ, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem, BNLMS, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Nie, Ji,Zhang, De-Wen,Tie, Cai,et al. G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection[J]. BIOSENSORS & BIOELECTRONICS,2014,56:237-242.
APA Nie, Ji,Zhang, De-Wen,Tie, Cai,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2014).G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection.BIOSENSORS & BIOELECTRONICS,56,237-242.
MLA Nie, Ji,et al."G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection".BIOSENSORS & BIOELECTRONICS 56(2014):237-242.

入库方式: OAI收割

来源:化学研究所

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