G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection
文献类型：期刊论文
| 作者 | Nie, Ji; Zhang, De-Wen; Tie, Cai; Zhou, Ying-Lin; Zhang, Xin-Xiang |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2014-06-15 |
| 卷号 | 56页码:237-242 |
| 关键词 | G-quadruplex Dna Detection Isothermal Exponential Amplification Label-free Colorimetry |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2014.01.032 |
| 英文摘要 | A novel G-quadruplex based two-stage isothermal exponential amplification reaction (GQ-EXPAR) was developed for label-free DNA calorimetric detection in this work. The exponential amplified trigger DNA in the first stage can convert into G-quadruplex sequence EAD2 by a linear amplification circuit in the second stage. Created EAD2 can form G-quadruplex/hemin DNAzyme to act as a direct signal readout element. The GQ-EXPAR combines the exponential amplification of DNA sequence and the peroxidase-mimicking DNAzyme induced signal amplification, which achieves tandem dual-amplification. Taking advantages of isothermal incubation, this label-free homogeneous assay obviates the need of thermal cycling. As no complex synthesis or extra downstream operation is needed, the whole easy handling procedure can be finished in no more than 1 h. This assay allows the sensing of the model DNA with the limit of detection to be 2.5 pM. Moreover, it demonstrates good discrimination of mismatched sequences. The strategy has also been successfully implemented to sensitively detect Tay Sachs genetic disorder mutant. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000333781000037 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51281]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Xin-Xiang |
| 作者单位 | Peking Univ, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem, BNLMS, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, Ji,Zhang, De-Wen,Tie, Cai,et al. G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection[J]. BIOSENSORS & BIOELECTRONICS,2014,56:237-242. |
| APA | Nie, Ji,Zhang, De-Wen,Tie, Cai,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2014).G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection.BIOSENSORS & BIOELECTRONICS,56,237-242. |
| MLA | Nie, Ji,et al."G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection".BIOSENSORS & BIOELECTRONICS 56(2014):237-242. |
入库方式： OAI收割
来源：化学研究所
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