Development of new chiral ligand exchange capillary electrophoresis system with amino acid ionic liquids ligands and its application in studying the kinetics of L-amino acid oxidase
文献类型：期刊论文
| 作者 | Sun, Bingbing1,3; Mu, Xiaoyu1,2; Qi, Li1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2014-04-22 |
| 卷号 | 821页码:97-102 |
| 关键词 | Amino Acid Ionic Liquid Chiral Ligand Exchange Capillary Electrophoresis L-amino Acid Oxidase Enzyme Kinetics |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2014.03.009 |
| 英文摘要 | New kinds of amino acid ionic liquids (AAILs) with pyridinium as cations and L-lysine (L-Lys) as anion have been developed as the available chiral ligands coordinated with Zn(II) in chiral ligand-exchange capillary electrophoresis (CLE-CE). Four kinds of AAILs, including [1-ethylpyridinium][L-lysine], [1-butylpyridinium][L-lysine], [1-hexylpyridinium][L-lysine] and 1-[octylpyridinium][L-lysine], were successfully synthesized and characterized by nuclear magnetic resonance and mass spectrometry. Compared with other AAILs, the best chiral separation of Dns-D, L-amino acids could be achieved when [1-ethylpyridinium][L-lysine] was chosen as the chiral ligand. It has been found that after investigating the influence of key factors on the separation efficiency, such as pH of buffer solution, the ratio of Zn(II) to ligand and complex concentration, eight pairs of Dns-D, L-AAs enantiomers could be baseline separated and three pairs were partly separated under the optimum conditions. The proposed CLE-CE method also exhibited favorable quantitative analysis property of Dns-D, L-Met with good linearity (r(2) = 0.998) and favorable repeatability (RSD <= 1.5%). Furthermore, the CLE-CE system was applied in investigating the kinetic contents of L-amino acid oxidase, which implied that the proposed system has the potential in studying the enzymatic reaction mechanism. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000333807600010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51345]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Bingbing,Mu, Xiaoyu,Qi, Li. Development of new chiral ligand exchange capillary electrophoresis system with amino acid ionic liquids ligands and its application in studying the kinetics of L-amino acid oxidase[J]. ANALYTICA CHIMICA ACTA,2014,821:97-102. |
| APA | Sun, Bingbing,Mu, Xiaoyu,&Qi, Li.(2014).Development of new chiral ligand exchange capillary electrophoresis system with amino acid ionic liquids ligands and its application in studying the kinetics of L-amino acid oxidase.ANALYTICA CHIMICA ACTA,821,97-102. |
| MLA | Sun, Bingbing,et al."Development of new chiral ligand exchange capillary electrophoresis system with amino acid ionic liquids ligands and its application in studying the kinetics of L-amino acid oxidase".ANALYTICA CHIMICA ACTA 821(2014):97-102. |
入库方式： OAI收割
来源：化学研究所
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