Associated Analysis of DNA Methylation for Cancer Detection Using CCP-Based FRET Technique
文献类型：期刊论文
| 作者 | Zhang, Jiangyan1; Xing, Baoling2; Song, Jinzhao1; Zhang, Feng2; Nie, Chenyao1; Jiao, Lian2; Liu, Libing1; Lv, Fengting1; Wang, Shu1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-01-07 |
| 卷号 | 86期号:1页码:346-350 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac402720g |
| 英文摘要 | This paper describes an associated analysis method of DNA methylation for the detection of cancer using an optically amplifying cationic conjugated polymer (CCP, poly{(1,4-phenylene)-2,7-[9,9-bis(6'-N,N,N-trimethyl ammonium)-hexyl fluorene] dibromide)}. Genomic DNA is digested by methylation-sensitive restriction endonuclease, followed by PCR amplification to incorporate fluorescein-labeled dNTP. Only methylated DNA can be amplified by PCR, and the methylation level is detected through fluorescence resonance energy transfer (FRET) between CCP and fluorescein that is incorporated into the PCR product. The methylation levels of RASSF1A, OPCML, and HOXA9 promoters of 35 ovarian cancer samples and 11 normal samples were assayed. In accordance with the degree of methylation levels, they are clustered to three sections and assigned a value. Through an associated analysis, we acquired a threshold for cancer detection with a sensitivity of 85.7%. The assay takes about 20 h to obtain the detection results and shows great potential as a useful tool for diagnostic and screening of cancer. |
| 语种 | 英语 |
| WOS记录号 | WOS:000329548700029 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51353]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100190, Peoples R China 2.Changzhou Women & Children Hlth Hosp, Dept Pathol, Changzhou 213003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiangyan,Xing, Baoling,Song, Jinzhao,et al. Associated Analysis of DNA Methylation for Cancer Detection Using CCP-Based FRET Technique[J]. ANALYTICAL CHEMISTRY,2014,86(1):346-350. |
| APA | Zhang, Jiangyan.,Xing, Baoling.,Song, Jinzhao.,Zhang, Feng.,Nie, Chenyao.,...&Wang, Shu.(2014).Associated Analysis of DNA Methylation for Cancer Detection Using CCP-Based FRET Technique.ANALYTICAL CHEMISTRY,86(1),346-350. |
| MLA | Zhang, Jiangyan,et al."Associated Analysis of DNA Methylation for Cancer Detection Using CCP-Based FRET Technique".ANALYTICAL CHEMISTRY 86.1(2014):346-350. |
入库方式： OAI收割
来源：化学研究所
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