Activity enhancement of G-quadruplex/hemin DNAzyme by spermine
文献类型：期刊论文
| 作者 | Qi, Cui1,2; Zhang, Nan1,2; Yan, Jingli1,2; Liu, Xiangjun1; Bing, Tao1; Mei, Hongcheng1,2; Shangguan, Dihua1 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2014 |
| 卷号 | 4期号:3页码:1441-1448 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c3ra45429k |
| 英文摘要 | Biogenic polyamines participate in regulating gene expression, activating DNA synthesis and facilitating DNA-protein interaction through interaction with DNA/RNA. The interaction of polyamines with G-quadruplexes (G4s) has been reported to modulate the structure of G4s. In this paper, we investigate the effects of polyamines on one of the properties of G4s, G4/hemin peroxidase. Three polyamines (spermine, spermidine and putrescine) are found to have positive effects on different G4/hemin DNAzymes, in which spermine exhibits the strongest enhancement efficiency. CD and UV/Vis spectral analysis suggests two reasons for the strong activity enhancement: first, spermine protects hemin from rapid degradation by H2O2; second, spermine condenses the G4 structures and provides a favorable microenvironment for the catalytic reaction. Since G4/hemin DNAzymes have been extensively applied in various chemical sensors and biosensors, this finding would be helpful for the design of G4/hemin based sensors and widen the application range of this kind of DNAzyme. |
| 语种 | 英语 |
| WOS记录号 | WOS:000327868400060 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51527]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shangguan, Dihua |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Cui,Zhang, Nan,Yan, Jingli,et al. Activity enhancement of G-quadruplex/hemin DNAzyme by spermine[J]. RSC ADVANCES,2014,4(3):1441-1448. |
| APA | Qi, Cui.,Zhang, Nan.,Yan, Jingli.,Liu, Xiangjun.,Bing, Tao.,...&Shangguan, Dihua.(2014).Activity enhancement of G-quadruplex/hemin DNAzyme by spermine.RSC ADVANCES,4(3),1441-1448. |
| MLA | Qi, Cui,et al."Activity enhancement of G-quadruplex/hemin DNAzyme by spermine".RSC ADVANCES 4.3(2014):1441-1448. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。