Evaluation of the toxicity of food additive silica nanoparticles on gastrointestinal cells
文献类型：期刊论文
| 作者 | Yang, Yi-Xin1; Song, Zheng-Mei1; Cheng, Bin1; Xiang, Kun1; Chen, Xin-Xin1; Liu, Jia-Hui2; Cao, Aoneng1; Wang, Yanli1; Liu, Yuanfang1,2; Wang, Haifang1 |
| 刊名 | JOURNAL OF APPLIED TOXICOLOGY
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 34期号:4页码:424-435 |
| 关键词 | Silica Nanoparticles Food Additive Gastrointestinal Cells Cytotoxicity Nanotoxicity |
| ISSN号 | 0260-437X |
| DOI | 10.1002/jat.2962 |
| 英文摘要 | Silica nanoparticles (NPs) have been widely used in food products as an additive; however, their toxicity and safety to the human body and the environment still remain unclear. As a food additive, silica NPs firstly enter the human gastrointestinal tract along with food, thus their gastrointestinal toxicity deserves thorough study. Herein, we evaluated the toxicity of food additive silica NPs to cells originating from the gastrointestinal tract. Four silica NP samples were introduced to human gastric epithelial cell GES-1 and colorectal adenocarcinoma cell Caco-2 to investigate the effect of silica sample, exposure dose and exposure period on the morphology, viability and membrane integrity of cells. The cell uptake, cellular reactive oxygen species (ROS) level, cell cycle and apoptosis were determined to reveal the toxicity mechanism. The results indicate that all four silica NPs are safe for both GES-1 and Caco-2 cells after 24-h exposure at a concentration lower than 100 mu gml(-1). At a higher concentration and longer exposure period, silica NPs do not induce the apoptosis/necrosis of cells, but arrest cell cycle and inhibit the cell growth. Notably, silica NPs do not pass through the Caco-2 cell monolayer after 4-h contact, indicating the low potential of silica NPs to cross the gastrointestinal tract in vivo. Our findings indicate that silica NPs could be used as a safe food additive, but more investigations, such as long-term in vivo exposure, are necessary in future studies. Copyright (c) 2013 John Wiley & Sons, Ltd. |
| 语种 | 英语 |
| WOS记录号 | WOS:000330314400011 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/51915]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Haifang |
| 作者单位 | 1.Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yi-Xin,Song, Zheng-Mei,Cheng, Bin,et al. Evaluation of the toxicity of food additive silica nanoparticles on gastrointestinal cells[J]. JOURNAL OF APPLIED TOXICOLOGY,2014,34(4):424-435. |
| APA | Yang, Yi-Xin.,Song, Zheng-Mei.,Cheng, Bin.,Xiang, Kun.,Chen, Xin-Xin.,...&Wang, Haifang.(2014).Evaluation of the toxicity of food additive silica nanoparticles on gastrointestinal cells.JOURNAL OF APPLIED TOXICOLOGY,34(4),424-435. |
| MLA | Yang, Yi-Xin,et al."Evaluation of the toxicity of food additive silica nanoparticles on gastrointestinal cells".JOURNAL OF APPLIED TOXICOLOGY 34.4(2014):424-435. |
入库方式： OAI收割
来源：化学研究所
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