Construction of a D-Amino Acid Oxidase Reactor Based on Magnetic Nanoparticles Modified by a Reactive Polymer and Its Application in Screening Enzyme Inhibitors
文献类型：期刊论文
| 作者 | Mu, Xiaoyu1,2; Qiao, Juan1; Qi, Li1; Liu, Ying3; Ma, Huimin1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2014-08-13 |
| 卷号 | 6期号:15页码:12979-12987 |
| 关键词 | Enzyme Immobilization D-amino Acid Oxidase Magnetic Nanoparticles Polymer Screening Inhibitors |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/am502901b |
| 英文摘要 | Developing facile and high-throughput methods for exploring pharmacological inhibitors of D-amino acid oxidase (DAAO) has triggered increasing interest. In this work, DAAO was immobilized on the magnetic nanoparticles, which were modified by a biocompatible reactive polymer, poly(glycidyl methacrylate) (PGMA) via an atom transfer radical polymerization technique. Interestingly, the enzyme immobilization process was greatly promoted with the assistance of a lithium perchlorate catalyst. Meanwhile, a new amino acid ionic liquid (AAIL) was successfully synthesized and employed as the efficient chiral ligand in a chiral ligand exchange capillary electrophoresis (CLE-CE) system for chiral separation of amino acids (AAs) and quantitation of methionine, which was selected as the substrate of DAAO. Then, the apparent Michaelis-Menten constants in the enzyme system were determined with the proposed CLE-CE method. The prepared DAAO-PGMA-Fe3O4 nanoparticles exhibited excellent reusability and good stability. Moreover, the enzyme reactor was successfully applied in screening DAAO inhibitors. These results demonstrated that the enzyme could be efficiently immobilized on the polymer-grafted magnetic nanoparticles and that the obtained enzyme reactor has great potential in screening enzyme inhibitors, further offering new insight into monitoring the relevant diseases. |
| 语种 | 英语 |
| WOS记录号 | WOS:000340446300140 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52011]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Minzu Univ China, Coll Life & Environm Sci, Beijing 100018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Xiaoyu,Qiao, Juan,Qi, Li,et al. Construction of a D-Amino Acid Oxidase Reactor Based on Magnetic Nanoparticles Modified by a Reactive Polymer and Its Application in Screening Enzyme Inhibitors[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(15):12979-12987. |
| APA | Mu, Xiaoyu,Qiao, Juan,Qi, Li,Liu, Ying,&Ma, Huimin.(2014).Construction of a D-Amino Acid Oxidase Reactor Based on Magnetic Nanoparticles Modified by a Reactive Polymer and Its Application in Screening Enzyme Inhibitors.ACS APPLIED MATERIALS & INTERFACES,6(15),12979-12987. |
| MLA | Mu, Xiaoyu,et al."Construction of a D-Amino Acid Oxidase Reactor Based on Magnetic Nanoparticles Modified by a Reactive Polymer and Its Application in Screening Enzyme Inhibitors".ACS APPLIED MATERIALS & INTERFACES 6.15(2014):12979-12987. |
入库方式： OAI收割
来源：化学研究所
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