DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer
文献类型：期刊论文
| 作者 | Wang, Yuanyuan1; Luo, Yun1; Bing, Tao2; Chen, Zheng1; Lu, Minhua1; Zhang, Nan2; Shangguan, Dihua2; Gao, Xin1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-06-23 |
| 卷号 | 9期号:6 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0100243 |
| 英文摘要 | Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa. |
| 语种 | 英语 |
| WOS记录号 | WOS:000338917900035 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52099]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shangguan, Dihua |
| 作者单位 | 1.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 510275, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yuanyuan,Luo, Yun,Bing, Tao,et al. DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer[J]. PLOS ONE,2014,9(6). |
| APA | Wang, Yuanyuan.,Luo, Yun.,Bing, Tao.,Chen, Zheng.,Lu, Minhua.,...&Gao, Xin.(2014).DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer.PLOS ONE,9(6). |
| MLA | Wang, Yuanyuan,et al."DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer".PLOS ONE 9.6(2014). |
入库方式： OAI收割
来源：化学研究所
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