A Label-Free Gold Nanocluster Fluorescent Probe for Protease Activity Monitoring
文献类型：期刊论文
| 作者 | Zhang, Jiajing1,2; Zhang, Zhen1; Nie, Xin2; Zhang, Zhenjiang2; Wu, Xiaochun2; Chen, Chunying2; Fang, Xiaohong1 |
| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 14期号:6页码:4029-4035 |
| 关键词 | Gold Nanoclusters Fluorescence Protease Detection Quenching Mechanism |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2014.8873 |
| 英文摘要 | Water soluble BSA-stabilized gold nanoclusters (Au NCs) were synthesized with a simple one-pot procedure. The as-prepared Au NCs were able to emit intensive red fluorescence under the excitation of ultraviolet light, and the fluorescence could be quenched by enzymatic hydrolysis. In this contribution, BSA-stabilized Au NCs as novel fluorescent probes were successfully utilized for the detection and real-time monitoring of proteolytic activity of trypsin and chymotrypsin. High performance liquid chromatography-inductively coupled plasma mass spectrometry, X-ray photoelectron spectroscopy, and X-ray absorption fine structure were performed to investigate the quenching mechanism, and the results indicated that BSA scaffold degradation caused by enzymatic proteolysis led to the decrease in fluorescence intensity. Furthermore, this method would be potentially extended to the detection of other enzymes with Au NCs stabilized by different biomolecules. |
| 语种 | 英语 |
| WOS记录号 | WOS:000332339500004 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52285]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Chunying |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiajing,Zhang, Zhen,Nie, Xin,et al. A Label-Free Gold Nanocluster Fluorescent Probe for Protease Activity Monitoring[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2014,14(6):4029-4035. |
| APA | Zhang, Jiajing.,Zhang, Zhen.,Nie, Xin.,Zhang, Zhenjiang.,Wu, Xiaochun.,...&Fang, Xiaohong.(2014).A Label-Free Gold Nanocluster Fluorescent Probe for Protease Activity Monitoring.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,14(6),4029-4035. |
| MLA | Zhang, Jiajing,et al."A Label-Free Gold Nanocluster Fluorescent Probe for Protease Activity Monitoring".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 14.6(2014):4029-4035. |
入库方式： OAI收割
来源：化学研究所
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