Preparation of a novel polymer monolith with functional polymer brushes by two-step atom-transfer radical polymerization for trypsin immobilization
文献类型：期刊论文
| 作者 | Li, Nan1,2; Zheng, Wei1,2; Shen, Ying1,2; Qi, Li1; Li, Yaping1,2; Qiao, Juan1; Wang, Fuyi1; Chen, Yi1 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 37期号:23页码:3411-3417 |
| ISSN号 | 1615-9306 |
| 关键词 | Atom-transfer Radical Polymerization Immobilized Reactor Porous Polymer Monolith Surface Grafting Trypsin |
| DOI | 10.1002/jssc.201400794 |
| 英文摘要 | Novel porous polymer monoliths grafted with poly{oligo[(ethylene glycol) methacrylate]-co-glycidyl methacrylate} brushes were fabricated via two-step atom-transfer radical polymerization and used as a trypsin-based reactor in a continuous flow system. This is the first time that atom-transfer radical polymerization technique was utilized to design and construct polymer monolith bioreactor. The prepared monoliths possessed excellent permeability, providing fast mass transfer for enzymatic reaction. More importantly, surface properties, which were modulated via surface-initiated atom-transfer radical polymerization, were found to have a great effect on bioreactor activities based on Michaelis-Menten studies. Furthermore, three model proteins were digested by the monolith bioreactor to a larger degree within dramatically reduced time (50 s), about 900 times faster than that by free trypsin (12 h). The proposed method provided a platform to prepare porous monoliths with desired surface properties for immobilizing various enzymes. |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000345829900002 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52323]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Nan,Zheng, Wei,Shen, Ying,et al. Preparation of a novel polymer monolith with functional polymer brushes by two-step atom-transfer radical polymerization for trypsin immobilization[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(23):3411-3417. |
| APA | Li, Nan.,Zheng, Wei.,Shen, Ying.,Qi, Li.,Li, Yaping.,...&Chen, Yi.(2014).Preparation of a novel polymer monolith with functional polymer brushes by two-step atom-transfer radical polymerization for trypsin immobilization.JOURNAL OF SEPARATION SCIENCE,37(23),3411-3417. |
| MLA | Li, Nan,et al."Preparation of a novel polymer monolith with functional polymer brushes by two-step atom-transfer radical polymerization for trypsin immobilization".JOURNAL OF SEPARATION SCIENCE 37.23(2014):3411-3417. |
入库方式： OAI收割
来源：化学研究所
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