Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin
文献类型：期刊论文
| 作者 | Yuan, Yuan1; Jiang, Chao3; Liu, Libing2; Yu, Shulin1; Cui, Zhanhu1; Chen, Min1; Lin, Shufang1; Wang, Shu2; Huang, Luqi1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-06-23 |
| 卷号 | 4 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep05395 |
| 英文摘要 | In this work, a rapid (within 4-5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products. |
| 语种 | 英语 |
| WOS记录号 | WOS:000337888600009 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52365]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Libing |
| 作者单位 | 1.Chinese Acad Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Daodi Herbs, Beijing 100700, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Beijing Normal Univ, Beijing Key Lab Protect & Applicat Chinese Med Re, Beijing 100875, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Yuan,Jiang, Chao,Liu, Libing,et al. Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Yuan, Yuan.,Jiang, Chao.,Liu, Libing.,Yu, Shulin.,Cui, Zhanhu.,...&Huang, Luqi.(2014).Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin.SCIENTIFIC REPORTS,4. |
| MLA | Yuan, Yuan,et al."Convenient, Sensitive and High-Throughput Method for Screening Botanic Origin".SCIENTIFIC REPORTS 4(2014). |
入库方式： OAI收割
来源：化学研究所
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