A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design
文献类型：期刊论文
| 作者 | Zhou, Zhenpeng1; Huang, Hongduan1; Chen, Yang1; Liu, Feng1; Huang, Cheng Zhi2; Li, Na1 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2014-02-15 |
| 卷号 | 52页码:367-373 |
| 关键词 | Metal-enhanced Fluorescence Molecular Beacon Distance-dependence Mercuric Ion |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2013.09.013 |
| 英文摘要 | A new metal-enhanced fluorescence (MEF) based platform was developed on the basis of distance-dependent fluorescence quenching-enhancement effect, which combined the easiness of Ag-thiol chemistry with the MEF property of noble-metal structures as well as the molecular beacon design. For the given sized AgNPs, the fluorescence enhancement factor was found to increase with a d(6) dependency in agreement with fluorescence resonance energy transfer mechanism at shorter distance and decrease with a d(-3) dependency in agreement with plasmonic enhancement mechanism at longer distance between the fluorophore and the AgNP surface. As a proof of concept, the platform was demonstrated by a sensitive detection of mercuric ions, using thymine-containing molecular beacon to tune silver nanoparticle (AgNP)-enhanced fluorescence. Mercuric ions were detected via formation of a thymine-mercuric-thymine structure to open the hairpin, facilitating fluorescence recovery and AgNP enhancement to yield a limit of detection of 1 nM, which is well below the U.S. Environmental Protection Agency regulation of the Maximum Contaminant Level Goal (10 nM) in drinking water. Since the AgNP functioned as not only a quencher to reduce the reagent blank signal but also an enhancement substrate to increase fluorescence of the open hairpin when target mercuric ions were present, the quenching-enhancement strategy can greatly improve the detection sensitivity and can in principle be a universal approach for various targets when combined with molecular beacon design. (C) 2013 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000328095400056 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52441]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ,Beijing Natl Lab Mol Sci, Inst Analyt Chem,Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China 2.Southwest Univ, Coll Pharmaceut Sci, Educ Minist, Key Lab Luminescence & Real Time Anal, Chongqing 400715, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhenpeng,Huang, Hongduan,Chen, Yang,et al. A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design[J]. BIOSENSORS & BIOELECTRONICS,2014,52:367-373. |
| APA | Zhou, Zhenpeng,Huang, Hongduan,Chen, Yang,Liu, Feng,Huang, Cheng Zhi,&Li, Na.(2014).A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design.BIOSENSORS & BIOELECTRONICS,52,367-373. |
| MLA | Zhou, Zhenpeng,et al."A distance-dependent metal-enhanced fluorescence sensing platform based on molecular beacon design".BIOSENSORS & BIOELECTRONICS 52(2014):367-373. |
入库方式： OAI收割
来源：化学研究所
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