Effects of Loops, Flanking Bases and Monovalent Cations on the Structures of G-Quadruplexes
文献类型：期刊论文
| 作者 | Chang Tianjun1; Li Gang1; Zhao Kexu1; Ban Lin1; Bian Wenxiu1; Bing Tao2; Shangguan Dihua2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2014-12-10 |
| 卷号 | 35期号:12页码:2556-2562 |
| 关键词 | G-quadruplex Topological Structure Dna Self-assembly Multimer |
| ISSN号 | 0251-0790 |
| DOI | 10.7503/cjeu20140408 |
| 英文摘要 | G-rich tandem repeats sequences are able to fold into structures called G-quadruplexes(G4s). G4s have complicated 'topological structures,-which closely depend on the sequences and the solution conditions. In this work, the effects of loops, flanking bases and monovalent cations on the structure of G4s were investigated by gel electrophoresis, circular dichroism spectra and UV-Melting. The results showed that G4 forming sequences with short loops were favorable to form parallel structure in K+ solution, and the parallel G4s without flanking bases were able to further form G4 multimers, but the anti-parallel or mixed parallel/anti-parallel G4s were not. The monovalent cations had an influence on the adopted structures; K+ was more favorable for the formation of parallel G4 and G4 multimers than NH4+ and Na+. The addition of non-guanine bases to the terminals of parallel G4s, or the transformation of the parallel G4s to non-parallel structures by changing the cations, could inhibit the formation of G4 multimers. Furthermore, loop length could affect the thermo-stability of G4s; G4s with short loops had higher thermo-stability. The G4 multimers showed lower thermo-stability than G4 monomer, and their size and amount became smaller with the temperature increase. These results suggest that G4s with short loops and no flanking bases fold into intramolecular parallel structures firstly, and then form multimers through end stacking. The flanking bases and loops located on the G-quartets at 3' or 5' terminal hinder the stacking interactions, and inhibit the multimer assembly. Our results may offer useful clues for the structure and function studies of G4s. |
| 语种 | 英语 |
| WOS记录号 | WOS:000347669400011 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52507]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chang Tianjun |
| 作者单位 | 1.Henan Polytech Univ, Inst Resources & Environm, Jiaozuo 454000, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang Tianjun,Li Gang,Zhao Kexu,et al. Effects of Loops, Flanking Bases and Monovalent Cations on the Structures of G-Quadruplexes[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2014,35(12):2556-2562. |
| APA | Chang Tianjun.,Li Gang.,Zhao Kexu.,Ban Lin.,Bian Wenxiu.,...&Shangguan Dihua.(2014).Effects of Loops, Flanking Bases and Monovalent Cations on the Structures of G-Quadruplexes.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,35(12),2556-2562. |
| MLA | Chang Tianjun,et al."Effects of Loops, Flanking Bases and Monovalent Cations on the Structures of G-Quadruplexes".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 35.12(2014):2556-2562. |
入库方式： OAI收割
来源：化学研究所
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