A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency
文献类型：期刊论文
| 作者 | Piao, Ji-Gang1,2; Yan, Jun-Jie1,2; Wang, Mo-Zhen1,2; Wu, De-Cheng3; You, Ye-Zi1,2 |
| 刊名 | BIOMATERIALS SCIENCE
 |
| 出版日期 | 2014 |
| 卷号 | 2期号:3页码:390-398 |
| ISSN号 | 2047-4830 |
| DOI | 10.1039/c3bm60204d |
| 英文摘要 | Disulfide-exchange was found to cross-link the polyplex of disulfide-containing poly(amido amine) and pDNA with heating of the polyplex solution over a short time. The cross-linked polyplexes based on disulfide-containing poly(amido amine) have excellent stability under physiological salt conditions, and have significantly enhanced transfection activity in the serum media compared to non-cross-linked polyplexes. In vivo, ICR mice were injected with the polyplex through the tail vein, the results show that the transfection efficiency of the cross-linked polyplex is higher than that of the non-cross-linked variety. Furthermore, the polyplex containing Cy5 labelled DNA was also injected into the mice to illustrate the stability and distribution of the polyplex, cross-linked polyplexes show a much brighter luminescence than the non-cross-linked ones. This method does not need a cross-linker or catalyst, and there are no impurities produced, it may be an elegant approach to resolve the dilemma of in vivo application of a DNA polyplex, with excellent stability whilst in circulation and a rapid unpacking of the polyplex inside the cells. |
| 语种 | 英语 |
| WOS记录号 | WOS:000330797800011 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52623]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Piao, Ji-Gang |
| 作者单位 | 1.Univ Sci & Technol China, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Polymer Sci & Engn, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Piao, Ji-Gang,Yan, Jun-Jie,Wang, Mo-Zhen,et al. A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency[J]. BIOMATERIALS SCIENCE,2014,2(3):390-398. |
| APA | Piao, Ji-Gang,Yan, Jun-Jie,Wang, Mo-Zhen,Wu, De-Cheng,&You, Ye-Zi.(2014).A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency.BIOMATERIALS SCIENCE,2(3),390-398. |
| MLA | Piao, Ji-Gang,et al."A new method to cross-link a polyplex for enhancing in vivo stability and transfection efficiency".BIOMATERIALS SCIENCE 2.3(2014):390-398. |
入库方式： OAI收割
来源：化学研究所
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