Location and function of the high-affinity chloride in the oxygen-evolving complex - Implications from comparing studies on Cl-/Br-/I--substituted photosystem II prepared using two different methods
文献类型：期刊论文
| 作者 | Wang, Luan; Zhang, Chunxi; Zhao, Jingquan |
| 刊名 | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
 |
| 出版日期 | 2014-09-05 |
| 卷号 | 138页码:249-255 |
| 关键词 | Photosystem Ii Oxygen-evolving Complex High-affinity Chloride Br-/i- Substitution Electron Paramagnetic Resonance |
| ISSN号 | 1011-1344 |
| DOI | 10.1016/j.jphotobiol.2014.05.021 |
| 英文摘要 | The high-affinity chloride ion (Cl-) is known to play a key role in water oxidation in photosystem II (PSII). Recent crystallographic studies revealed two Cl- binding sites in PSII. To examine whether these two C1 ions are correlated to the high-affinity Cl-, we prepared Cl-/Br-/I--substituted PSII samples from both higher plants and cyanobacteria by using two different protocols: one was the method used in the crystallographic study (Type 1) and the other was a method developed recently to ensure the efficient replacement of Cl- (Type 2). While only minor effects were observed in the Type 1 preparation, efficient Br-/I(-)substitution by the Type 2 protocol led to significant changes in the EPR properties of the oxygenevolving complex (OEC) and the Tyr(z), as well as in oxygen-evolving activities. These results are discussed in terms of the binding site of the high-affinity Cl- relative to the two Cl- ions revealed by the recent X-ray structural data. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000340987500029 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52685]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Chunxi |
| 作者单位 | Chinese Acad Sci, Inst Chem, Lab Photochem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Luan,Zhang, Chunxi,Zhao, Jingquan. Location and function of the high-affinity chloride in the oxygen-evolving complex - Implications from comparing studies on Cl-/Br-/I--substituted photosystem II prepared using two different methods[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2014,138:249-255. |
| APA | Wang, Luan,Zhang, Chunxi,&Zhao, Jingquan.(2014).Location and function of the high-affinity chloride in the oxygen-evolving complex - Implications from comparing studies on Cl-/Br-/I--substituted photosystem II prepared using two different methods.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,138,249-255. |
| MLA | Wang, Luan,et al."Location and function of the high-affinity chloride in the oxygen-evolving complex - Implications from comparing studies on Cl-/Br-/I--substituted photosystem II prepared using two different methods".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 138(2014):249-255. |
入库方式： OAI收割
来源：化学研究所
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