Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment
文献类型：期刊论文
| 作者 | Meng, Lingchen; Fang, Ziyuan; Lin, Jian; Li, Meixian; Zhu, Zhiwei |
| 刊名 | TALANTA
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 121页码:205-209 |
| 关键词 | Capillary Electrophoresis Copper Edta Hela Cells |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2014.01.009 |
| 英文摘要 | A new separation system of capillary electrophoresis (CE1) for the highly sensitive determination of copper was established by using ethylenediaminetetraacetic acid (EDTA) as a complexing agent and employing cetyltrimethylammonium chloride (CTAC) as a capillary inner wall modifier. Benefitted from the combination of field-enhanced sample injection (FESI) method, a limit of detection (LOD) of 2.7 nM was obtained, which was much lower than that of the conventional methods. This made it possible to determine trace copper in HeLa cell only by a simple cell extraction (CE2) treatment. Two copper-extraction methods acid-hydrolysis and freeze-thaw were compared. Limited by the requirement of low ion strength in FESI, only the extract using freeze-thaw could be successfully applied in the determination. The effectiveness assessment of this CE2-FESI method was adopted by inductively coupled plasma-atomic emission spectrometry (ICP-AES) as a gold standard. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000333791500027 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52733]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhu, Zhiwei |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Lingchen,Fang, Ziyuan,Lin, Jian,et al. Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment[J]. TALANTA,2014,121:205-209. |
| APA | Meng, Lingchen,Fang, Ziyuan,Lin, Jian,Li, Meixian,&Zhu, Zhiwei.(2014).Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment.TALANTA,121,205-209. |
| MLA | Meng, Lingchen,et al."Highly sensitive determination of copper in HeLa cell using capillary electrophoresis combined with a simple cell extraction treatment".TALANTA 121(2014):205-209. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。