Lysosomal pH Rise during Heat Shock Monitored by a Lysosome-Targeting Near-Infrared Ratiometric Fluorescent Probe
文献类型：期刊论文
| 作者 | Wan, Qiongqiong; Chen, Suming; Shi, Wen; Li, Lihong; Ma, Huimin |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2014-10-06 |
| 卷号 | 53期号:41页码:10916-10920 |
| 关键词 | Dyes/pigments Fluorescent Probes Heat Shock Imaging Agents Lysosomal Ph |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201405742 |
| 英文摘要 | Heat stroke is a life-threatening condition, featuring a high body temperature and malfunction of many organ systems. The relationship between heat shock and lysosomes is poorly understood, mainly because of the lack of a suitable research approach. Herein, by incorporating morpholine into a stable hemicyanine skeleton, we develop a new lysosome-targeting near-infrared ratiometric pH probe. In combination with fluorescence imaging, we show for the first time that the lysosomal pH value increases but never decreases during heat shock, which might result from lysosomal membrane permeabilization. We also demonstrate that this lysosomal pH rise is irreversible in living cells. Moreover, the probe is easy to synthesize, and shows superior overall analytical performance as compared to the existing commercial ones. This enhanced performance may enable it to be widely used in more lysosomal models of living cells and in further revealing the mechanisms underlying heat-related pathology. |
| 语种 | 英语 |
| WOS记录号 | WOS:000343750300013 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52889]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, Huimin |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Qiongqiong,Chen, Suming,Shi, Wen,et al. Lysosomal pH Rise during Heat Shock Monitored by a Lysosome-Targeting Near-Infrared Ratiometric Fluorescent Probe[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2014,53(41):10916-10920. |
| APA | Wan, Qiongqiong,Chen, Suming,Shi, Wen,Li, Lihong,&Ma, Huimin.(2014).Lysosomal pH Rise during Heat Shock Monitored by a Lysosome-Targeting Near-Infrared Ratiometric Fluorescent Probe.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,53(41),10916-10920. |
| MLA | Wan, Qiongqiong,et al."Lysosomal pH Rise during Heat Shock Monitored by a Lysosome-Targeting Near-Infrared Ratiometric Fluorescent Probe".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53.41(2014):10916-10920. |
入库方式： OAI收割
来源：化学研究所
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