Fast and quantitative differentiation of single-base mismatched DNA by initial reaction rate of catalytic hairpin assembly
文献类型：期刊论文
| 作者 | Li, Chenxi1; Li, Yixin1; Xu, Xiao1; Wang, Xinyi2; Chen, Yang1; Yang, Xiaoda2; Liu, Feng1; Li, Na1 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2014-10-15 |
| 卷号 | 60页码:57-63 |
| 关键词 | Dna Single-base Mismatch Catalytic Hairpin Assembly Initial Reaction Rate |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2014.04.007 |
| 英文摘要 | The widely used catalytic hairpin assembly (CHA) amplification strategy generally needs several hours to accomplish one measurement based on the prevailingly used maximum intensity detection mode, making it less practical for assays where high throughput or speed is desired. To make the best use of the kinetic specificity of toehold domain for circuit reaction initiation, we developed a mathematical model and proposed an initial reaction rate detection mode to quantitatively differentiate the single-base mismatch. Using the kinetic mode, assay time can be reduced substantially to 10 min for one measurement with the comparable sensitivity and single-base mismatch differentiating ability as were obtained by the maximum intensity detection mode. This initial reaction rate based approach not only provided a fast and quantitative differentiation of single-base mismatch, but also helped in-depth understanding of the CHA system, which will be beneficial to the design of highly sensitive and specific toehold-mediated hybridization reactions. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000337863900008 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/52955]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Peking Univ, Inst Analyt Chem, Coll Chem & Mol Engn, BNLMS,Key Lab Bioorgan Chem & Mol Engn,Minist Edu, Beijing 100871, Peoples R China 2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chenxi,Li, Yixin,Xu, Xiao,et al. Fast and quantitative differentiation of single-base mismatched DNA by initial reaction rate of catalytic hairpin assembly[J]. BIOSENSORS & BIOELECTRONICS,2014,60:57-63. |
| APA | Li, Chenxi.,Li, Yixin.,Xu, Xiao.,Wang, Xinyi.,Chen, Yang.,...&Li, Na.(2014).Fast and quantitative differentiation of single-base mismatched DNA by initial reaction rate of catalytic hairpin assembly.BIOSENSORS & BIOELECTRONICS,60,57-63. |
| MLA | Li, Chenxi,et al."Fast and quantitative differentiation of single-base mismatched DNA by initial reaction rate of catalytic hairpin assembly".BIOSENSORS & BIOELECTRONICS 60(2014):57-63. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。