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Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection

文献类型：期刊论文

作者Zhao, Qiuling1,2; Zhang, Zhen1; Xu, Li1; Xia, Ted1; Li, Nan1; Liu, Jianli1; Fang, Xiaohong1
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2014-05-01
卷号406期号:12页码:2949-2955
关键词Aptamer Assay Exonuclease i Colorimetric Detection Atp L-argininamide
ISSN号1618-2642
DOI10.1007/s00216-014-7705-z
英文摘要A novel enzyme-linked aptamer assay (ELAA) with the aid of Exonuclease I (Exo I) for colorimetric detection of small molecules was developed. The fluorescein isothiocyanate (FITC)-labeled aptamer was integrated into a double-stranded DNA (dsDNA). In the presence of target, the binding of aptamer with target protected the aptamer from Exo I degradation, which resulted in the FITC tag remaining on the aptamer. Then, the anti-FITC-HRP conjugate was used to produce an optically observable signal. By monitoring the color change, we were able to detect two model molecules, ATP and L-argininamide, with high selectivity and high sensitivity even in the serum matrix. It is expected to be a simple and general ELAA method with wide applicability.
语种英语
WOS记录号WOS:000334431300021
出版者SPRINGER HEIDELBERG
源URL[http://ir.iccas.ac.cn/handle/121111/53147]  
专题中国科学院化学研究所
通讯作者Fang, Xiaohong
作者单位1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Liaoning Tech Univ, Basic Course Dept, Huludao 125105, Liaoning, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Qiuling,Zhang, Zhen,Xu, Li,et al. Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(12):2949-2955.
APA Zhao, Qiuling.,Zhang, Zhen.,Xu, Li.,Xia, Ted.,Li, Nan.,...&Fang, Xiaohong.(2014).Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,406(12),2949-2955.
MLA Zhao, Qiuling,et al."Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.12(2014):2949-2955.

入库方式： OAI收割

来源：化学研究所

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