Glucose affinity measurement by surface plasmon resonance with borate polymer binding
文献类型：期刊论文
| 作者 | Li, Dachao1; Yang, Di1; Yang, Jia1; Lin, Yuan2; Sun, Yingjuan2; Yu, Haixia1; Xu, Kexin1 |
| 刊名 | SENSORS AND ACTUATORS A-PHYSICAL
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 222页码:58-66 |
| 关键词 | Continuous Glucose Monitoring Surface Plasmon Resonance Affinity Measurement Borate Polymer Layer-by-layer Self-assembly |
| ISSN号 | 0924-4247 |
| DOI | 10.1016/j.sna.2014.10.039 |
| 英文摘要 | A novel surface plasmon resonance (SPR) sensor bound to the borate polymer PAA-ran-PAAPBA through a layer-by-layer method was proposed for the determination of glucose concentration. In contrast to the enzyme electrode sensor, the use of optical refractive index sensing to detect glucose concentration eliminates the measurement drift caused by bioelectricity when the sensor is implanted into subcutaneous tissue; in addition, a borate polymer was used to replace the glucose oxidase (GOD) enzyme and it does not consume the glucose molecule during measurement through the affinity reaction between the polymer and glucose. In this study, the layer-by-layer self-assembly method was used to immobilize the borate polymer on the surface of the SPR sensor. The effects of the number of layers are discussed in the manuscript, and the regenerability, reproducibility, and stability of the SPR sensor were evaluated. Almost all of the studies are performed under specific alkaline conditions (usually close to or higher than the pKa of PBA). And for the future application in vivo, we further investigated glucose detection at physiological conditions. The measurement resolution of the sensor bound to 12 polymer layers at physiological conditions was 1 mg/dL, and the R-squared value of the glucose concentration-Delta RU fitting curve within 1-1000 mg/dL was as high as 0.998, which indicates that this measurement may form the foundation of an implantable device for the continuous measurement of glucose concentration. (C) 2014 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000349573400008 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/53359]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Dachao |
| 作者单位 | 1.Tianjin Univ, State Key Lab Precis Measuring Technol & Instrume, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Dachao,Yang, Di,Yang, Jia,et al. Glucose affinity measurement by surface plasmon resonance with borate polymer binding[J]. SENSORS AND ACTUATORS A-PHYSICAL,2015,222:58-66. |
| APA | Li, Dachao.,Yang, Di.,Yang, Jia.,Lin, Yuan.,Sun, Yingjuan.,...&Xu, Kexin.(2015).Glucose affinity measurement by surface plasmon resonance with borate polymer binding.SENSORS AND ACTUATORS A-PHYSICAL,222,58-66. |
| MLA | Li, Dachao,et al."Glucose affinity measurement by surface plasmon resonance with borate polymer binding".SENSORS AND ACTUATORS A-PHYSICAL 222(2015):58-66. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。