Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization
文献类型：期刊论文
| 作者 | Wang, Qinli1; Zhao, Yuanyuan1; Luo, Wangxi2; Li, Ruili1; He, Qihua3; Fang, Xiaohong2; De Michele, Roberto4,5; Ast, Cindy4; von Wiren, Nicolaus6; Lin, Jinxing1 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2013-08-06 |
| 卷号 | 110期号:32页码:13204-13209 |
| 关键词 | Va-tirfm Fcs |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1301160110 |
| 英文摘要 | Ammonium is a preferred source of nitrogen for plants but is toxic at high levels. Plant ammonium transporters (AMTs) play an essential role in NH4+ uptake, but the mechanism by which AMTs are regulated remains unclear. To study how AMTs are regulated in the presence of ammonium, we used variable-angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy for single-particle fluorescence imaging of EGFP-tagged AMT1;3 on the plasma membrane of Arabidopsis root cells at various ammonium levels. We demonstrated that AMT1;3-EGFP dynamically appeared and disappeared on the plasma membrane as moving fluorescent spots in low oligomeric states under N-deprived and N-sufficient conditions. Under external high-ammonium stress, however, AMT1;3-EGFPs were found to amass into clusters, which were then internalized into the cytoplasm. A similar phenomenon also occurred in the glutamine synthetase mutant gln1;2 background. Single-particle analysis of AMT1;3-EGFPs in the clathrin heavy chain 2 mutant (chc2 mutant) and Flotllin1 artificial microRNA (Flot1 amiRNA) backgrounds, together with chemical inhibitor treatments, demonstrated that the endocytosis of AMT1;3 clusters induced by high-ammonium stress could occur mainly through clathrin-mediated endocytic pathways, but the contribution of microdomain-associated endocytic pathway cannot be excluded in the internalization. Our results revealed that the clustering and endocytosis of AMT1;3 provides an effective mechanism by which plant cells can avoid accumulation of toxic levels of ammonium by eliminating active AMT1;3 from the plasma membrane. |
| 语种 | 英语 |
| WOS记录号 | WOS:000322771100079 |
| 出版者 | NATL ACAD SCIENCES |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/53775]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lin, Jinxing |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China 4.Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA 5.Natl Res Council Italy, Inst Plant Genet, I-90123 Palermo, Italy 6.Leibniz Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany |
| 推荐引用方式 GB/T 7714 | Wang, Qinli,Zhao, Yuanyuan,Luo, Wangxi,et al. Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(32):13204-13209. |
| APA | Wang, Qinli.,Zhao, Yuanyuan.,Luo, Wangxi.,Li, Ruili.,He, Qihua.,...&Lin, Jinxing.(2013).Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(32),13204-13209. |
| MLA | Wang, Qinli,et al."Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.32(2013):13204-13209. |
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来源：化学研究所
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