Quantitative Analysis of Oligosaccharides Derived from Sulfated Glycosaminoglycans by Nanodiamond-Based Affinity Purification and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
文献类型：期刊论文
| 作者 | Hsieh, Chih-Chien1,5; Guo, Jiun You2; Hung, Shain-Un2; Chen, Rui3,4; Nie, Zongxiu3,4; Chang, Huan-Cheng1,5; Wu, Chih-Che2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013-05-07 |
| 卷号 | 85期号:9页码:4342-4349 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac3034097 |
| 英文摘要 | Degraded fragments of sulfated glycosaminoglycans (GAGs) are key reporters for profiling the burden of mucopolysaccharidosis (MPS) disease at baseline and during therapy. Here, we present a high-throughput assay, which combines microwave-assisted degradation, solid-phase affinity purification, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), for quantitative analysis of sulfated oligosaccharides in biological samples. First, sulfated oligosaccharides such as chondroitin-4-sulfate (CS) were efficiently isolated from highly diluted solutions or spiked artificial cerebrospinal fluid (aCSF) using polyarginine-coated nanodiamonds (PA-coated NDs) as affinity sorbents. Next, they were degraded to disaccharides through microwave assisted methanolysis or enzymatic digestion for subsequent MALDI-TOF MS analysis. The reaction times for GAG depolymerization were significantly reduced from a few hours to less than 7 min under the microwave irradiation. Deuterium-labeled internal standards were then mixed with the CS-derived disaccharides for quantitative analysis by MALDI-TOF MS using the N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) matrix. The new assay is facile, specific (with distinct chlorine isotope trait markers), sensitive (with a detection limit of similar to 70 pg), and potentially useful for clinical diagnosis of MPS. |
| 语种 | 英语 |
| WOS记录号 | WOS:000318756100023 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/53777]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wu, Chih-Che |
| 作者单位 | 1.Natl Taiwan Normal Univ, Dept Chem, Taipei 106, Taiwan 2.Natl Chi Nan Univ, Dept Appl Chem, Puli 545, Nantou, Taiwan 3.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 4.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 5.Acad Sinica, Inst Atom & Mol Sci, Taipei 106, Taiwan |
| 推荐引用方式 GB/T 7714 | Hsieh, Chih-Chien,Guo, Jiun You,Hung, Shain-Un,et al. Quantitative Analysis of Oligosaccharides Derived from Sulfated Glycosaminoglycans by Nanodiamond-Based Affinity Purification and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2013,85(9):4342-4349. |
| APA | Hsieh, Chih-Chien.,Guo, Jiun You.,Hung, Shain-Un.,Chen, Rui.,Nie, Zongxiu.,...&Wu, Chih-Che.(2013).Quantitative Analysis of Oligosaccharides Derived from Sulfated Glycosaminoglycans by Nanodiamond-Based Affinity Purification and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.ANALYTICAL CHEMISTRY,85(9),4342-4349. |
| MLA | Hsieh, Chih-Chien,et al."Quantitative Analysis of Oligosaccharides Derived from Sulfated Glycosaminoglycans by Nanodiamond-Based Affinity Purification and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry".ANALYTICAL CHEMISTRY 85.9(2013):4342-4349. |
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来源：化学研究所
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