A chiral ligand exchange CE system for monitoring inhibitory effect of kojic acid on tyrosinase
文献类型：期刊论文
| 作者 | Sun, Bing-bing1,3; Qi, Li1; Mu, Xiao-yu1,2; Qiao, Juan1; Wang, Ming-lin3 |
| 刊名 | TALANTA
 |
| 出版日期 | 2013-11-15 |
| 卷号 | 116页码:1121-1125 |
| 关键词 | Capillary Electrophoresis Chiral Ligand Exchange Kojic Acid Tyrosinase Inhibitor |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2013.08.028 |
| 英文摘要 | A facile chiral ligand exchange capillary electrophoresis (CLE-CE) system with Zn(II)-L-alanine as the chiral selector in the presence of beta-cyclodextrin has been developed for enantioseparation of dansyl amino acids. The influence of the key factors, such as buffer pH, the ratio of Zn (II) to ligand, the concentration of beta-cyclodextrin and the concentration of the complex, were investigated in detail when D, L-Tyr and D, L-Thr were selected as the model analytes. The proposed method showed favorable quantitative analysis property of dansyl D, L-tyrosine with good linearity (r(2) >= 0.999) and reproducibility (RSD <= 3.8%), then, it was applied in studying the activity of tyrosinase through the determination of L-tyrosine concentration variation after being incubated with the enzyme. Further, the inhibitory efficiency of kojic acid and soy sauce on the tyrosinase was investigated. The IC50 of kojic acid obtained from the sigmoidal inhibitory curve was 21.35 mu M. The results imply that the proposed CLE-CE system has the potential in exploring the activity of enzyme and screening the inhibitors of enzyme. (C) 2013 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000328176000161 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/53791]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Bing-bing,Qi, Li,Mu, Xiao-yu,et al. A chiral ligand exchange CE system for monitoring inhibitory effect of kojic acid on tyrosinase[J]. TALANTA,2013,116:1121-1125. |
| APA | Sun, Bing-bing,Qi, Li,Mu, Xiao-yu,Qiao, Juan,&Wang, Ming-lin.(2013).A chiral ligand exchange CE system for monitoring inhibitory effect of kojic acid on tyrosinase.TALANTA,116,1121-1125. |
| MLA | Sun, Bing-bing,et al."A chiral ligand exchange CE system for monitoring inhibitory effect of kojic acid on tyrosinase".TALANTA 116(2013):1121-1125. |
入库方式： OAI收割
来源：化学研究所
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