A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules
文献类型：期刊论文
| 作者 | Gu, Xinggui1; Zhang, Guanxin1; Wang, Zhuo2; Liu, Wenwen2; Xiao, Le2; Zhang, Deqing1 |
| 刊名 | ANALYST
 |
| 出版日期 | 2013 |
| 卷号 | 138期号:8页码:2427-2431 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c3an36784c |
| 英文摘要 | Based on the consideration that compound 1 with a -PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL(-1) can be assayed with compound 1. Moreover, compound 1 has been successfully applied for ALP assay in living cells. Also, compound 1 is useful for screening inhibitors of ALP. |
| 语种 | 英语 |
| WOS记录号 | WOS:000316222300030 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/53839]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Guanxin |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Natl Ctr Nanosci & Technol, Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Xinggui,Zhang, Guanxin,Wang, Zhuo,et al. A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules[J]. ANALYST,2013,138(8):2427-2431. |
| APA | Gu, Xinggui,Zhang, Guanxin,Wang, Zhuo,Liu, Wenwen,Xiao, Le,&Zhang, Deqing.(2013).A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules.ANALYST,138(8),2427-2431. |
| MLA | Gu, Xinggui,et al."A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules".ANALYST 138.8(2013):2427-2431. |
入库方式： OAI收割
来源：化学研究所
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