Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP
文献类型：期刊论文
| 作者 | Zhao, Min1; Yang, Lingye1; Zhang, Ruichen1; Dong, Jie1; Dong, Haifeng2; Wen, Yongqiang2; Zhan, Xiaowei3; Wang, Ge1; Lu, YunFeng1; Wang, Guojie1 |
| 刊名 | POLYMER
 |
| 出版日期 | 2013-01-08 |
| 卷号 | 54期号:1页码:297-302 |
| 关键词 | Hybridization Fluorescence Polyelectrolytes |
| ISSN号 | 0032-3861 |
| DOI | 10.1016/j.polymer.2012.11.029 |
| 英文摘要 | A novel pyrene-labeled polyelectrolyte (Py-PDMAEMA(+)) has been prepared by atom transfer radical polymerization (ATRP) for detection of DNA hybridization. The electrostatic and hydrophobic interactions between Py-PDMAEMA(+) and ssDNA (hairpin or linear DNA) kept them forming a complex probe which could be used as a turn-off fluorescent sensor. The fluorescence intensity of the probe decreased upon adding the complementary strand, since the pyrene moiety could be intercalated into the duplex and thus the fluorescence quenching by nucleotide bases was strengthened. The proposed intercalation mode was confirmed by the circular dichroism spectra and the fluorescence quenching study with iodide. This pyrene-labeled polyelectrolyte combined with ssDNA may establish a novel fluorescence sensing system for DNA hybridization. (C) 2012 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000322356000035 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/53991]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Guojie |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Min,Yang, Lingye,Zhang, Ruichen,et al. Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP[J]. POLYMER,2013,54(1):297-302. |
| APA | Zhao, Min.,Yang, Lingye.,Zhang, Ruichen.,Dong, Jie.,Dong, Haifeng.,...&Wang, Guojie.(2013).Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP.POLYMER,54(1),297-302. |
| MLA | Zhao, Min,et al."Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP".POLYMER 54.1(2013):297-302. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。