Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation
文献类型：期刊论文
| 作者 | Nie, Chenyao; Zhu, Chunlei; Feng, Liheng; Lv, Fengting; Liu, Libing; Wang, Shu |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2013-06-12 |
| 卷号 | 5期号:11页码:4549-4554 |
| 关键词 | Conjugated Polymers Dna Alkylation Plasmid Gfp Gene Regulation Protein Expression |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/am4001985 |
| 英文摘要 | A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized, PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. Combining the features of alkylating function, multivalent binding sites, and fluorescent characteristics, PFP-Cbl provides a new insight in the area of gene regulation and extends the new applications of conjugated polymers (CPs). |
| 语种 | 英语 |
| WOS记录号 | WOS:000320484000010 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54023]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lv, Fengting |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, Chenyao,Zhu, Chunlei,Feng, Liheng,et al. Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(11):4549-4554. |
| APA | Nie, Chenyao,Zhu, Chunlei,Feng, Liheng,Lv, Fengting,Liu, Libing,&Wang, Shu.(2013).Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation.ACS APPLIED MATERIALS & INTERFACES,5(11),4549-4554. |
| MLA | Nie, Chenyao,et al."Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation".ACS APPLIED MATERIALS & INTERFACES 5.11(2013):4549-4554. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。