Inhibition of Hepatitis C Virus Infection by DNA Aptamer against Envelope Protein
文献类型：期刊论文
| 作者 | Yang, Darong1,2,3; Meng, Xianghe1; Yu, Qinqin1; Xu, Li4; Long, Ying1; Liu, Bin1; Fang, Xiaohong4; Zhu, Haizhen1,2,3 |
| 刊名 | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 57期号:10页码:4937-4944 |
| ISSN号 | 0066-4804 |
| DOI | 10.1128/AAC.00897-13 |
| 英文摘要 | Hepatitis C virus (HCV) envelope protein (E1E2) is essential for virus binding to host cells. Aptamers have been demonstrated to have strong promising applications in drug development. In the current study, a cDNA fragment encoding the entire E1E2 gene of HCV was cloned. E1E2 protein was expressed and purified. Aptamers for E1E2 were selected by the method of selective evolution of ligands by exponential enrichment (SELEX), and the antiviral actions of the aptamers were examined. The mechanism of their antiviral activity was investigated. The data show that selected aptamers for E1E2 specifically recognize the recombinant E1E2 protein and E1E2 protein from HCV-infected cells. CD81 protein blocks the binding of aptamer E1E2-6 to E1E2 protein. Aptamers against E1E2 inhibit HCV infection in an infectious cell culture system although they have no effect on HCV replication in a replicon cell line. Beta interferon (IFN-beta) and IFN-stimulated genes (ISGs) are not induced in virus-infected hepatocytes with aptamer treatment, suggesting that E1E2-specific aptamers do not induce innate immunity. E2 protein is essential for the inhibition of HCV infection by aptamer E1E2-6, and the aptamer binding sites are located in E2. Q412R within E1E2 is the major resistance substitution identified. The data indicate that an aptamer against E1E2 exerts its antiviral effects through inhibition of virus binding to host cells. Aptamers against E1E2 can be used with envelope protein to understand the mechanisms of HCV entry and fusion. The aptamers may hold promise for development as therapeutic drugs for hepatitis C patients. |
| 语种 | 英语 |
| WOS记录号 | WOS:000324480300042 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54173]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhu, Haizhen |
| 作者单位 | 1.Hunan Univ, Dept Mol Med, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China 2.Cent S Univ, Xiangya Med Sch, Hunan Prov Tumor Hosp, Translat Med Res Ctr Liver Canc,Ctr Canc Prevent, Changsha, Hunan, Peoples R China 3.Cent S Univ, Xiangya Med Sch, Affiliated Tumor Hosp, Changsha, Hunan, Peoples R China 4.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Darong,Meng, Xianghe,Yu, Qinqin,et al. Inhibition of Hepatitis C Virus Infection by DNA Aptamer against Envelope Protein[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2013,57(10):4937-4944. |
| APA | Yang, Darong.,Meng, Xianghe.,Yu, Qinqin.,Xu, Li.,Long, Ying.,...&Zhu, Haizhen.(2013).Inhibition of Hepatitis C Virus Infection by DNA Aptamer against Envelope Protein.ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,57(10),4937-4944. |
| MLA | Yang, Darong,et al."Inhibition of Hepatitis C Virus Infection by DNA Aptamer against Envelope Protein".ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 57.10(2013):4937-4944. |
入库方式： OAI收割
来源：化学研究所
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