Highly sensitive, selective and reusable mercury(II) ion sensor based on a ssDNA-functionalized photonic crystal film
文献类型：期刊论文
| 作者 | Zhang, Yuqi1; Gao, Loujun1; Wen, Liping2; Heng, Liping2; Song, Yanlin2 |
| 刊名 | PHYSICAL CHEMISTRY CHEMICAL PHYSICS
 |
| 出版日期 | 2013 |
| 卷号 | 15期号:28页码:11943-11949 |
| ISSN号 | 1463-9076 |
| DOI | 10.1039/c3cp51324f |
| 英文摘要 | We have developed a highly sensitive, selective and reusable fluorescence sensor with photonic crystal (PC) films for mercury(II) ion detection, based on the Bragg reflection of PCs and formation of thymine-Hg2+-thymine (T-Hg2+-T) complexes. The T-rich single stranded DNA (ssDNA) labeled by a fluorophore was self-assembled on the surface of Au-sputtered PCs through Au-thiol binding, in which the DNA exists in a single stranded chain. The obtained ssDNA-functionalized PC films show a strong fluorescence emission derived from the Bragg reflection of PCs, because the fluorescence wavelength of ssDNA is in the range of the selected PC stopband. After reaction with Hg2+ ions, the conformation of ssDNA changes from the original single stranded chain to a folded hairpin structure due to the formation of T-Hg2+-T complexes. This leads to a fluorescence resonance energy transfer process between the fluorophore and the thin gold film, which results in significant fluorescence quenching. The sensitivity of the fluorescence detection, with a detection limit of 4 nM, can be obviously enhanced by the Bragg reflection of PCs compared to the control sample without PC structures. The prepared sensor is negligibly responsive to other metal ions. In addition, the sensor can also be easily regenerated and reused by decoupling the T-Hg2+-T base pairs using cysteine. As a result, a highly sensitive, selective and reusable Hg2+ ion sensor based on a ssDNA-functionalized PC film has been achieved, which will be of importance for the effective and practical detection of heavy metal ions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000321201500044 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54305]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Yuqi |
| 作者单位 | 1.Yanan Univ, Coll Chem & Chem Engn, Yanan 716000, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuqi,Gao, Loujun,Wen, Liping,et al. Highly sensitive, selective and reusable mercury(II) ion sensor based on a ssDNA-functionalized photonic crystal film[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2013,15(28):11943-11949. |
| APA | Zhang, Yuqi,Gao, Loujun,Wen, Liping,Heng, Liping,&Song, Yanlin.(2013).Highly sensitive, selective and reusable mercury(II) ion sensor based on a ssDNA-functionalized photonic crystal film.PHYSICAL CHEMISTRY CHEMICAL PHYSICS,15(28),11943-11949. |
| MLA | Zhang, Yuqi,et al."Highly sensitive, selective and reusable mercury(II) ion sensor based on a ssDNA-functionalized photonic crystal film".PHYSICAL CHEMISTRY CHEMICAL PHYSICS 15.28(2013):11943-11949. |
入库方式： OAI收割
来源：化学研究所
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