Nitroreductase Detection and Hypoxic Tumor Cell Imaging by a Designed Sensitive and Selective Fluorescent Probe, 7-[(5-Nitrofuran-2-yl)methoxy]-3H-phenoxazin-3-one
文献类型：期刊论文
| 作者 | Li, Zhao; Li, Xiaohua; Gao, Xinghui; Zhang, Yangyang; Shi, Wen; Ma, Huimin |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013-04-16 |
| 卷号 | 85期号:8页码:3926-3932 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac400750r |
| 英文摘要 | A highly selective and sensitive fluorescence probe, 7-[(5-nitrofuran-2-yl)methoxy]-3H-phenoxazin-3-one (1), is developed for imaging the hypoxic status of tumor cells via the indirect detection of nitroreductase. The detection mechanism is based on the fact that nitroreductase can selectively catalyze the reduction of the nitro group in 1 to a hydroxylamine or amino group in the presence of reduced nicotinamide adenine dinucleotide as an electron donor that is indispensable, followed by the 1,6-rearrangement-elimination and the release of resorufin. As a result, the reaction produces a distinct color and fluorescence change from almost colorless and nonfluorescent to pink and strong red fluorescence. The fluorescence increase of probe 1 at lambda(550/585) (nm) is directly proportional to the concentration of nitroreductase in the range of 15-300 ng/mL, with a detection limit of 0.27 ng/mL. The ready reduction of the nitro group in 1 under hypcodc conditions leads to the establishment of a sensitive and selective fluorescence method for imaging the hypoxic status of tumor cells, and with this method Hela and A549 cells under normoxic and hypoxic conditions (even for different extents of hypoxia) can be differentiated successfully. This method is simple and may be useful for the imaging of disease-relevant hypoxia. |
| 语种 | 英语 |
| WOS记录号 | WOS:000317794800021 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54469]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Xiaohua |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhao,Li, Xiaohua,Gao, Xinghui,et al. Nitroreductase Detection and Hypoxic Tumor Cell Imaging by a Designed Sensitive and Selective Fluorescent Probe, 7-[(5-Nitrofuran-2-yl)methoxy]-3H-phenoxazin-3-one[J]. ANALYTICAL CHEMISTRY,2013,85(8):3926-3932. |
| APA | Li, Zhao,Li, Xiaohua,Gao, Xinghui,Zhang, Yangyang,Shi, Wen,&Ma, Huimin.(2013).Nitroreductase Detection and Hypoxic Tumor Cell Imaging by a Designed Sensitive and Selective Fluorescent Probe, 7-[(5-Nitrofuran-2-yl)methoxy]-3H-phenoxazin-3-one.ANALYTICAL CHEMISTRY,85(8),3926-3932. |
| MLA | Li, Zhao,et al."Nitroreductase Detection and Hypoxic Tumor Cell Imaging by a Designed Sensitive and Selective Fluorescent Probe, 7-[(5-Nitrofuran-2-yl)methoxy]-3H-phenoxazin-3-one".ANALYTICAL CHEMISTRY 85.8(2013):3926-3932. |
入库方式： OAI收割
来源：化学研究所
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