One-step real time RT-PCR for detection of microRNAs
文献类型：期刊论文
| 作者 | Yan, Jingli1,2; Zhang, Nan1,2; Qi, Cui1,2; Liu, Xiangjun1; Shangguan, Dihua1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2013-06-15 |
| 卷号 | 110页码:190-195 |
| 关键词 | Microrna One-step Real Time Pcr Reverse Transcription |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2013.02.028 |
| 英文摘要 | Rapid and simple methods for microRNA (miRNA) detection are essential for biological research of miRNAs and clinical diagnosis. Here we describe a sensitive and specific real time RT-PCR (also RT-qPCR) method for miRNA quantification. The whole detection process including reverse transcription and PCR is performed in one PCR tube by a one-step operation on a real-time PCR system. The results display a wide linear range from 0.1 amol to 10 fmol with a detection limit of 12.6 zmol for miRNA let-7a detection. Let-7a in small RNA samples extracted from tumor cells has been successfully detected by this method. This method is cost-effective, simple and rapid, and has the advantages in the high-throughput routing assay of given miRNAs, as well as in non-model research that has less specific kits and reagents. (C) 2013 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000319089500027 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54477]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shangguan, Dihua |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Being Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Jingli,Zhang, Nan,Qi, Cui,et al. One-step real time RT-PCR for detection of microRNAs[J]. TALANTA,2013,110:190-195. |
| APA | Yan, Jingli,Zhang, Nan,Qi, Cui,Liu, Xiangjun,&Shangguan, Dihua.(2013).One-step real time RT-PCR for detection of microRNAs.TALANTA,110,190-195. |
| MLA | Yan, Jingli,et al."One-step real time RT-PCR for detection of microRNAs".TALANTA 110(2013):190-195. |
入库方式： OAI收割
来源：化学研究所
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