G-quadruplex DNA aptamers for zeatin recognizing
文献类型：期刊论文
| 作者 | Qi, Cui1,2; Bing, Tao1; Mei, Hongcheng1,2; Yang, Xiaojuan1,2; Liu, Xiangjun1; Shangguan, Dihua1 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013-03-15 |
| 卷号 | 41页码:157-162 |
| 关键词 | Aptamer Zeatin Aptasensor Selex Graphene Oxide |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2012.08.004 |
| 英文摘要 | Zeatins, a major type of cytokinin, are ubiquitous in higher plants, and involve in regulating a wide range of developmental processes. The development of highly specific ligands to zeatins would be very useful in plant biological research. Here we describe a group of oligonucleotide ligands (aptamers) generated against trans-zeatin. The optimized aptamers possess strong affinity to trans-zeatin and trans-zeatin riboside (Kd=3-5 mu M), and relatively weak affinity (Kd=27-30 mu M) to cis-zeatin and dihydrozeatin. These aptamers adopt a hairpin-G-quadruplex structure for binding to zeatin. A fluorescence turn-on aptasensor based on graphene oxide (GO) was developed for the recognition of zeatins. The specificity assay of this aptasensor shows good response to zeatins, and no response to the adenine derivatives (analog of zeatins) abundantly existing in biological samples. These results show the great potential of these aptamers in chemical analysis and biological investigation of zeatins. (C) 2012 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000314191300020 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54521]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shangguan, Dihua |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Cui,Bing, Tao,Mei, Hongcheng,et al. G-quadruplex DNA aptamers for zeatin recognizing[J]. BIOSENSORS & BIOELECTRONICS,2013,41:157-162. |
| APA | Qi, Cui,Bing, Tao,Mei, Hongcheng,Yang, Xiaojuan,Liu, Xiangjun,&Shangguan, Dihua.(2013).G-quadruplex DNA aptamers for zeatin recognizing.BIOSENSORS & BIOELECTRONICS,41,157-162. |
| MLA | Qi, Cui,et al."G-quadruplex DNA aptamers for zeatin recognizing".BIOSENSORS & BIOELECTRONICS 41(2013):157-162. |
入库方式： OAI收割
来源：化学研究所
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