Isolation of anti-tumor compounds from the stem bark of Zanthoxylum ailanthoides Sieb. & Zucc. by silica gel column and counter-current chromatography
文献类型：期刊论文
| 作者 | Cao, Xue-li1; Xu, Jing1; Bai, Ge1; Zhang, Hong2,3; Liu, Yan2; Xiang, Jun-feng2; Tang, Ya-lin2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2013-06-15 |
| 卷号 | 929页码:6-10 |
| 关键词 | Antitumor Activity Coumarins Counter-current Chromatography (Ccc) Zanthoxylum Ailanthoides Sieb. & Zucc. Lignans |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2013.04.006 |
| 英文摘要 | Silica gel column chromatography combined with high performance counter-current chromatography (HPCCC) was employed for the separation of potential anti-tumor compounds from a petroleum ether fraction of a crude extract of Zanthoxylum ailanthoides Sieb. & Zucc. This traditional Chinese medicine was recently found to display high inhibitory activity against A-549 human cancer cells in vitro and Lewis lung cancer in vivo. A 75% aqueous ethanol extract of the stem bark of Z. ailanthoides was fractionated with petroleum ether, ethyl acetate and n-butanol. In this paper, the petroleum ether fraction was pre-separated by silica gel column chromatography with a petroleum ether-ethyl acetate gradient. Two fractions were further separated and purified by HPCCC using n-hexane-ethyl acetate-methanol-water (3:1:2:1, v/v) and petroleum-ethyl acetate-methanol-water (8:6:7:7, v/v). Finally, coumarins and lignans including luvangetin, xanthyletin, hinokinin and asarinin were isolated and identified by MS, H-1 and C-13 NMR. In total, 56 mg of xanthyletin (1), 140 mg of hinokinin (2), 850 mg of luvangetin (3) and 74 mg of asarinin (4) were obtained from approximately 50 g of petroleum ether extract, in 96.0%, 94.0%, 99.0% and 94.0% purity, respectively, as determined by HPLC. The separation method proved to be efficient, especially for those minor components. (C) 2013 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000320085300002 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54605]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cao, Xue-li |
| 作者单位 | 1.Beijing Technol & Business Univ, Dept Bioengn, Beijing 100048, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Xue-li,Xu, Jing,Bai, Ge,et al. Isolation of anti-tumor compounds from the stem bark of Zanthoxylum ailanthoides Sieb. & Zucc. by silica gel column and counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,929:6-10. |
| APA | Cao, Xue-li.,Xu, Jing.,Bai, Ge.,Zhang, Hong.,Liu, Yan.,...&Tang, Ya-lin.(2013).Isolation of anti-tumor compounds from the stem bark of Zanthoxylum ailanthoides Sieb. & Zucc. by silica gel column and counter-current chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,929,6-10. |
| MLA | Cao, Xue-li,et al."Isolation of anti-tumor compounds from the stem bark of Zanthoxylum ailanthoides Sieb. & Zucc. by silica gel column and counter-current chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 929(2013):6-10. |
入库方式： OAI收割
来源：化学研究所
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